Protein Variants | Comment | Organism |
---|---|---|
C130A | low activity with dihydroorotate, increasing activity above pH 8.0 with dihydrooxonate | Lactococcus lactis |
S175A | - |
Lactococcus lactis |
S175A | low activity with dihydroorotate, increasing activity above pH 8.0 with dihydrooxonate | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
family 2 enzyme | - |
Lactococcus lactis | - |
family 1 enzymes A and B | - |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-dihydroorotate + fumarate = orotate + succinate | cysteine and serine are active site base residues. One-site ping-pong mechanism with fumarate as electron acceptor | Escherichia coli | |
(S)-dihydroorotate + fumarate = orotate + succinate | cysteine and serine are active site base residues. One-site ping-pong mechanism with fumarate as electron acceptor | Lactococcus lactis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + acceptor | fumarate and ferricyanide tested as electron acceptors | Escherichia coli | orotate + reduced acceptor | - |
? | |
(S)-dihydroorotate + acceptor | fumarate and ferricyanide tested as electron acceptors | Lactococcus lactis | orotate + reduced acceptor | - |
? | |
dihydrooxonate + acceptor | fumarate and ferricyanide tested as electron acceptors | Escherichia coli | oxonate + reduced acceptor | - |
? | |
dihydrooxonate + acceptor | fumarate and ferricyanide tested as electron acceptors | Lactococcus lactis | oxonate + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
DHOD | - |
Escherichia coli |
DHOD | - |
Lactococcus lactis |