Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 0.175 mM restores inhibited enzyme after 3 min incubation to 90% of full activity | Lactobacillus delbrueckii subsp. bulgaricus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
HgCl2 | - |
Lactobacillus delbrueckii subsp. bulgaricus | |
Mercuric chloride | 40% inhibition after 15 min at 0.000045 mM | Lactobacillus delbrueckii subsp. bulgaricus | |
additional information | markable sensitivity to sulfhydryl inhibitors | Lactobacillus delbrueckii subsp. bulgaricus | |
N-ethylmaleimide | - |
Lactobacillus delbrueckii subsp. bulgaricus | |
Orotate | competitive to dihydroorotate in ferricyanide reduction assay | Lactobacillus delbrueckii subsp. bulgaricus | |
p-hydroxymercuribenzoate | 98% inhibition after 5 min at 0.00227 mM | Lactobacillus delbrueckii subsp. bulgaricus | |
Tiron | inhibits cytochrome c reduction and sulfite autoxidation | Lactobacillus delbrueckii subsp. bulgaricus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
S-dihydroorotate | with ferricyanide as electron acceptor | Lactobacillus delbrueckii subsp. bulgaricus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
57000 | - |
gel filtration | Lactobacillus delbrueckii subsp. bulgaricus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + O2 | Lactobacillus delbrueckii subsp. bulgaricus | pathway of pyrimidine biosynthesis, constitutive biosynthetic enzyme | orotate + H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactobacillus delbrueckii subsp. bulgaricus | - |
09, ATCC 13866 | - |
Purification (Comment) | Organism |
---|---|
preparative gel electrophoresis | Lactobacillus delbrueckii subsp. bulgaricus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(S)-dihydroorotate + fumarate = orotate + succinate | sulfhydryl groups suggested to be located near the active site, suggested that enzyme produces superoxide free radicals upon reaction with oxygen | Lactobacillus delbrueckii subsp. bulgaricus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-dihydroorotate + ferricyanide | ferricyanide usually used as electon acceptor in the assay | Lactobacillus delbrueckii subsp. bulgaricus | orotate + ferrocyanide | - |
? | |
(S)-dihydroorotate + O2 | ferricyanide, cytochrome c and 2,6-dichlorophenolindolphenol as alternate electron acceptor | Lactobacillus delbrueckii subsp. bulgaricus | orotate + H2O2 | - |
? | |
(S)-dihydroorotate + O2 | pathway of pyrimidine biosynthesis, constitutive biosynthetic enzyme | Lactobacillus delbrueckii subsp. bulgaricus | orotate + H2O2 | - |
? |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FMN | flavoprotein, enzyme contains 1 FMN as prosthetic group | Lactobacillus delbrueckii subsp. bulgaricus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
Orotate | - |
Lactobacillus delbrueckii subsp. bulgaricus |