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Literature summary for 1.3.98.1 extracted from

  • Taylor, W.H.; Taylor, C.D.; Taylor, M.L.
    Biosynthetic dihydroorotate dehydrogenase from Lactobacillus bulgaricus: partial characterization of the enzyme (1974), J. Bacteriol., 119, 98-105.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol 0.175 mM restores inhibited enzyme after 3 min incubation to 90% of full activity Lactobacillus delbrueckii subsp. bulgaricus

Inhibitors

Inhibitors Comment Organism Structure
HgCl2
-
Lactobacillus delbrueckii subsp. bulgaricus
Mercuric chloride 40% inhibition after 15 min at 0.000045 mM Lactobacillus delbrueckii subsp. bulgaricus
additional information markable sensitivity to sulfhydryl inhibitors Lactobacillus delbrueckii subsp. bulgaricus
N-ethylmaleimide
-
Lactobacillus delbrueckii subsp. bulgaricus
Orotate competitive to dihydroorotate in ferricyanide reduction assay Lactobacillus delbrueckii subsp. bulgaricus
p-hydroxymercuribenzoate 98% inhibition after 5 min at 0.00227 mM Lactobacillus delbrueckii subsp. bulgaricus
Tiron inhibits cytochrome c reduction and sulfite autoxidation Lactobacillus delbrueckii subsp. bulgaricus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.5
-
S-dihydroorotate with ferricyanide as electron acceptor Lactobacillus delbrueckii subsp. bulgaricus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
gel filtration Lactobacillus delbrueckii subsp. bulgaricus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-dihydroorotate + O2 Lactobacillus delbrueckii subsp. bulgaricus pathway of pyrimidine biosynthesis, constitutive biosynthetic enzyme orotate + H2O2
-
?

Organism

Organism UniProt Comment Textmining
Lactobacillus delbrueckii subsp. bulgaricus
-
09, ATCC 13866
-

Purification (Commentary)

Purification (Comment) Organism
preparative gel electrophoresis Lactobacillus delbrueckii subsp. bulgaricus

Reaction

Reaction Comment Organism Reaction ID
(S)-dihydroorotate + fumarate = orotate + succinate sulfhydryl groups suggested to be located near the active site, suggested that enzyme produces superoxide free radicals upon reaction with oxygen Lactobacillus delbrueckii subsp. bulgaricus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-dihydroorotate + ferricyanide ferricyanide usually used as electon acceptor in the assay Lactobacillus delbrueckii subsp. bulgaricus orotate + ferrocyanide
-
?
(S)-dihydroorotate + O2 ferricyanide, cytochrome c and 2,6-dichlorophenolindolphenol as alternate electron acceptor Lactobacillus delbrueckii subsp. bulgaricus orotate + H2O2
-
?
(S)-dihydroorotate + O2 pathway of pyrimidine biosynthesis, constitutive biosynthetic enzyme Lactobacillus delbrueckii subsp. bulgaricus orotate + H2O2
-
?

Cofactor

Cofactor Comment Organism Structure
FMN flavoprotein, enzyme contains 1 FMN as prosthetic group Lactobacillus delbrueckii subsp. bulgaricus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.1
-
Orotate
-
Lactobacillus delbrueckii subsp. bulgaricus