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Literature summary for 1.3.8.8 extracted from
- Recombinant adeno-associated virus-mediated gene delivery of long chain acyl coenzyme A dehydrogenase (LCAD) into LCAD-deficient mice (2008), J. Gene Med., 10, 1113-1123.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | LCAD gene replacement to the tibialis anterior muscle and livers of partially deficient LCAD mice is described, a surrogate model for human VLCAD deficiency | Mus musculus |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| a vector plasmid pCB-mLCAD for transfection of LCAD-deficient mice is used | Mus musculus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Mus musculus | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 44000 | - |
determined by SDS-PAGE and Western Blot analysis | Mus musculus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mus musculus | - |
mice heterozygous for a mutation in LCAD, strain B6.129S6-Acadltm1Uab Hsd | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| hepatocyte | - |
Mus musculus | - |
| tibialis anterior | muscle | Mus musculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.06037 | - |
LCAD enzyme activity in livers of partially deficient LCAD mice 8 weeks after injection of recombinant adeno-associated viral mLCAD vector via the hepatic portal vein | Mus musculus |
| 0.2546 | - |
LCAD enzyme activity in tibialis anterior muscle of LCAD-heterozygous deficient mice 10 weeks post-injection 1 x 10exp11 vg recombinant adeno-associated viral mLCAD | Mus musculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylheptanoyl-CoA + acceptor | activity assay | Mus musculus | ? | - |
? |  |
| palmitoyl-CoA + acceptor | activity assay | Mus musculus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LCAD | - |
Mus musculus |
| long chain acyl coenzyme A dehydrogenase | - |
Mus musculus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 32 | - |
activity assay | Mus musculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
activity assay | Mus musculus |
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Release 2023.1 (January 2023)
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