Literature summary for 1.3.8.8 extracted from

Eder, M.; Krautle, F.; Dong, Y.; Vock, P.; Kieweg, V.; Kim, J.J.; Strauss, A.W.; Ghisla, S.
Characterization of human and pig kidney long-chain-acyl-CoA dehydrogenases and their role in beta-oxidation (1997), Eur. J. Biochem., 245, 600-607.

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Cloned(Commentary)

Cloned (Comment)	Organism
expression in Escherichia coli, strain BL21(DE)	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
2-octynoyl-CoA	90% inhibition at 5 mol inhibitor/mol enzyme	Homo sapiens
2-octynoyl-CoA	65% inhibition at 5 mol inhibitor/mol enzyme	Sus scrofa

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0015	-	dodecanoyl-CoA	-	Sus scrofa
0.0022	-	decanoyl-CoA	-	Sus scrofa
0.0023	-	tetradecanoyl-CoA	-	Sus scrofa
0.0033	-	octanoyl-CoA	-	Sus scrofa
0.0036	-	palmitoyl-CoA	-	Sus scrofa
0.0037	-	stearoyl-CoA	-	Sus scrofa
0.0063	-	hexanoyl-CoA	-	Sus scrofa
0.007	-	dodecanoyl-CoA	-	Homo sapiens
0.008	-	octanoyl-CoA	-	Homo sapiens
0.008	-	stearoyl-CoA	-	Homo sapiens
0.009	-	electron-transfer flavoprotein	saturating dodecanoyl-CoA	Sus scrofa
0.01	-	decanoyl-CoA	-	Homo sapiens
0.01	-	tetradecanoyl-CoA	-	Homo sapiens
0.012	-	electron-transfer flavoprotein	saturating dodecanoyl-CoA, measured with ETF from pig kidney	Homo sapiens
0.014	-	palmitoyl-CoA	-	Homo sapiens
0.029	-	hexanoyl-CoA	-	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
44000	-	x * 44000, SDS-PAGE	Homo sapiens
44000	-	x * 44000, SDS-PAGE	Sus scrofa
180000	-	gel filtration	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-
Sus scrofa	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
40-80% ammonium sulfate precipitation, Q-Sepharose I, Q-Sepharose II, hydroxyapatite	Homo sapiens
Q-Sepharose, hydroxyapatite I, hydroxyapatite II	Sus scrofa

Source Tissue

Source Tissue	Comment	Organism	Textmining
kidney	-	Sus scrofa	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
15.27	-	purified enzyme, decanoyl-CoA	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
decanoyl-CoA + electron-transfer flavoprotein	-	Homo sapiens	2-decenoyl-CoA + reduced electron-transfer flavoprotein	-	?
decanoyl-CoA + electron-transfer flavoprotein	-	Sus scrofa	2-decenoyl-CoA + reduced electron-transfer flavoprotein	-	?
dodecanoyl-CoA + electron-transfer flavoprotein	-	Homo sapiens	2-dodecenoyl-CoA + reduced electron-transfer flavoprotein	-	?
dodecanoyl-CoA + electron-transfer flavoprotein	-	Sus scrofa	2-dodecenoyl-CoA + reduced electron-transfer flavoprotein	-	?
hexanoyl-CoA + electron-transfer flavoprotein	-	Homo sapiens	2-hexenoyl-CoA + reduced electron-transfer flavoprotein	-	?
hexanoyl-CoA + electron-transfer flavoprotein	-	Sus scrofa	2-hexenoyl-CoA + reduced electron-transfer flavoprotein	-	?
octanoyl-CoA + electron-transfer flavoprotein	-	Homo sapiens	2-octenoyl-CoA + reduced electron-transfer flavoprotein	-	?
octanoyl-CoA + electron-transfer flavoprotein	-	Sus scrofa	2-octenoyl-CoA + reduced electron-transfer flavoprotein	-	?
palmitoyl-CoA + electron-transfer flavoprotein	-	Homo sapiens	2-hexadecenoyl-CoA + reduced electron-transfer flavoprotein	-	?
palmitoyl-CoA + electron-transfer flavoprotein	-	Sus scrofa	2-hexadecenoyl-CoA + reduced electron-transfer flavoprotein	-	?
stearoyl-CoA + electron-transfer flavoprotein	-	Homo sapiens	2-octadecenoyl-CoA + reduced electron-transfer flavoprotein	-	?
stearoyl-CoA + electron-transfer flavoprotein	-	Sus scrofa	2-octadecenoyl-CoA + reduced electron-transfer flavoprotein	-	?
tetradecanoyl-CoA + electron-transfer flavoprotein	-	Homo sapiens	2-tetradecenoyl-CoA + reduced electron-transfer flavoprotein	-	?
tetradecanoyl-CoA + electron-transfer flavoprotein	-	Sus scrofa	2-tetradecenoyl-CoA + reduced electron-transfer flavoprotein	-	?

Subunits

Subunits	Comment	Organism
?	x * 44000, SDS-PAGE	Homo sapiens
?	x * 44000, SDS-PAGE	Sus scrofa

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Release 2023.1 (January 2023)