Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
Y375A | very low acyl-CoA dehydrogenase activity | Rattus norvegicus |
Y375E | inactive | Rattus norvegicus |
Y375F | very low acyl-CoA dehydrogenase activity | Rattus norvegicus |
Y375K | very low acyl-CoA dehydrogenase activity, mutant exhibits acyl-CoA oxidase activity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00084 | - |
octanoyl-CoA | mutant enzyme Y375K, in 50 mM potassium phosphate buffer, pH 7.6 | Rattus norvegicus | |
0.0023 | - |
octanoyl-CoA | wild type enzyme, in 50 mM potassium phosphate buffer, pH 7.6 | Rattus norvegicus | |
0.00242 | - |
octanoyl-CoA | mutant enzyme Y375F, in 50 mM potassium phosphate buffer, pH 7.6 | Rattus norvegicus | |
0.00684 | - |
octanoyl-CoA | mutant enzyme Y375A, in 50 mM potassium phosphate buffer, pH 7.6 | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Rattus norvegicus | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
SDS-PAGE | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
HiTrap chelating nickel affinity chromatography | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Storage Stability | Organism |
---|---|
-80°C, 6 months, remains stable | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acyl-CoA + acceptor | - |
Rattus norvegicus | 2,3-dehydroacyl-CoA + reduced acceptor | - |
? | |
hexanoyl-CoA + 2,6-dichloroindophenol | - |
Rattus norvegicus | 2-hexenoyl-CoA + reduced 2,6-dichloroindophenol | - |
? | |
octanoyl-CoA + 2,6-dichloroindophenol | optimal substrate for wild type enzyme | Rattus norvegicus | 2-octenoyl-CoA + reduced 2,6-dichloroindophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MCAD | - |
Rattus norvegicus |
medium chain acyl-CoA dehydrogenase | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.038 | - |
octanoyl-CoA | mutant enzyme Y375K, in 50 mM potassium phosphate buffer, pH 7.6 | Rattus norvegicus | |
0.22 | - |
octanoyl-CoA | mutant enzyme Y375A, in 50 mM potassium phosphate buffer, pH 7.6 | Rattus norvegicus | |
0.37 | - |
octanoyl-CoA | mutant enzyme Y375F, in 50 mM potassium phosphate buffer, pH 7.6 | Rattus norvegicus | |
3.3 | - |
octanoyl-CoA | wild type enzyme, in 50 mM potassium phosphate buffer, pH 7.6 | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rattus norvegicus |