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Literature summary for 1.3.8.7 extracted from

  • Kuchler, B.; Abdel-Ghany, A.G.; Bross, P.; Nandy, A.; Rasched, I.; Ghisla, S.
    Biochemical characterization of a variant human medium-chain acyl-CoA dehydrogenase with a disease-associated mutation localized in the active site (1999), Biochem. J., 337, 225-230.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of T168A mutant enzyme in Escherichia coli Homo sapiens

Protein Variants

Protein Variants Comment Organism
T168A thermostability is markedly decreased, 80% lowered activity in ferricinum assay Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
T168A mutation found in patients with medium-chain acyl-CoA deficiency
-

Purification (Commentary)

Purification (Comment) Organism
recombinant T168A mutant enzyme Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
octanoyl-CoA + recombinant beta-Y16L electron transferring flavoprotein
-
Homo sapiens trans-oct-2-enoyl-CoA + reduced recombinant beta-Y16L electron transferring flavoprotein
-
r
octanoyl-CoA + recombinant electron transferring flavoprotein
-
Homo sapiens trans-oct-2-enoyl-CoA + reduced recombinant electron transferring flavoprotein
-
r

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
41
-
wild-type enzyme: almost no loss of activity after 1 h at 41°C, T168A mutant enzyme: complete inactivation after 20 min at 41°C Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1
-
recombinant electron transferring flavoprotein T168A mutant enzyme Homo sapiens
1.67
-
recombinant beta-Y16L electron transferring flavoprotein T168A mutant enzyme Homo sapiens
14.7
-
recombinant electron transferring flavoprotein wild-type enzyme Homo sapiens
18.3
-
recombinant beta-Y16L electron transferring flavoprotein wild-type enzyme Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9 10 T168A mutant Homo sapiens
10
-
-
Homo sapiens

pH Range

pH Minimum pH Maximum Comment Organism
6 10 T168A mutant enzyme, approx. 20% of maximum activity at pH 6.0, sigmoidal increase between pH 5.5 and pH 9.0, approx. 80% of maximal activity at pH 7.8 Homo sapiens
7 10 approx. 20% of maximum activity at pH 7.5, sigmoidal increase between pH 7.0 and pH 10.0, approx. 80% of maximal activity at pH 9.0 Homo sapiens