Crystallization (Comment) | Organism |
---|---|
wild-type and E376G/T255E double mutant enzyme and enzyme substrate complexes, vapor diffusion method at 4°C using the sitting drop technique, 0.027 mg enzyme in 140 mM Tris-acetate, pH 7.0, 8% w/v polyethylene glycol 4000, the human enzyme structure is essentially the same as that of the pig enzyme | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Sus scrofa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
wild-type and E376G/T255E double mutant enzyme | Homo sapiens |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
a medium-chain acyl-CoA + electron-transfer flavoprotein = a medium-chain trans-2,3-dehydroacyl-CoA + reduced electron-transfer flavoprotein | the catalytic base responsible for the alpha-proton abstraction is E376 in medium-chain acyl-CoA dehydrogenase, while that in long-chain acyl-CoA dehydrogenase is E255 | Homo sapiens |