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Literature summary for 1.3.8.6 extracted from

  • Blazquez, B.; Carmona, M.; Garcia, J.L.; Diaz, E.
    Identification and analysis of a glutaryl-CoA dehydrogenase-encoding gene and its cognate transcriptional regulator from Azoarcus sp. CIB (2008), Environ. Microbiol., 10, 474-482.
    View publication on PubMed
Search for more literature for 1.3.8.6

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli D5alpha and MC4100 cells Azoarcus sp.
expressed in the Azoarcus sp. CIBdgcdR mutant strain Pseudomonas putida

Protein Variants

Protein Variants Comment Organism
additional information the CIBdgcdR mutant strain is unable to grow in pimelate, glutarate or benzoate as sole carbon sources Azoarcus sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 SDS-PAGE Azoarcus sp.

Organism

Organism UniProt Comment Textmining
Azoarcus sp. B0EVL5
-
-
Pseudomonas putida
-
-
-
Pseudomonas putida KT 2240
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pseudomonas putida
-
 Azoarcus sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture condition:benzoate-grown cell
-
 Pseudomonas putida
-
culture condition:benzoate-grown cell
-
 Azoarcus sp.
-
culture condition:pimelate-grown cell
-
 Pseudomonas putida
-
culture condition:pimelate-grown cell
-
 Azoarcus sp.
-
culture condition:pyruvate-grown cell
-
 Pseudomonas putida
-
culture condition:pyruvate-grown cell
-
 Azoarcus sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.028
-
 in cells grown in 0.2% (w/v) pyruvate Azoarcus sp.
0.169
-
 in cells grown in 3 mM pimelate Azoarcus sp.
0.19
-
 in cells grown in 0.2% (w/v) pyruvate and 3 mM pimelate Azoarcus sp.
0.216
-
 in cells grown in 3 mM benzoate Azoarcus sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glutaryl-CoA + ferricenium hexafluorophosphate
-
 Pseudomonas putida crotonyl-CoA + CO2 + ferrocenium hexafluorophosphate
-
 ?
glutaryl-CoA + ferricenium hexafluorophosphate
-
 Azoarcus sp. crotonyl-CoA + CO2 + ferrocenium hexafluorophosphate
-
 ?
glutaryl-CoA + ferricenium hexafluorophosphate
-
 Pseudomonas putida KT 2240 crotonyl-CoA + CO2 + ferrocenium hexafluorophosphate
-
 ?

Synonyms

Synonyms Comment Organism
GCDH
-
 Pseudomonas putida
GCDH
-
 Azoarcus sp.

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Pseudomonas putida
FAD
-
 Azoarcus sp.