Protein Variants | Comment | Organism |
---|---|---|
E370Q | the dienolate intermediate observed upon decarboxylation of glutaconyl-CoA can be detected with E370Q GCD but not with wild-type, because the crotonyl-CoA dienolate forms a charge-transfer complex with the oxidized FAD and the substitution of a glutamine residue for Glu370 prevents rapid protonation of the dienolate | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0047 | - |
glutaryl-CoA | at pH 6.5 | Homo sapiens | |
0.0081 | - |
glutaryl-CoA | at pH 7.6 | Homo sapiens | |
0.034 | - |
glutaryl-CoA | at pH 8.5 | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q92947 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaconyl-CoA + acceptor | - |
Homo sapiens | crotonyl-CoA + CO2 + reduced acceptor | - |
? | |
glutaryl-CoA + acceptor | - |
Homo sapiens | crotonoyl-CoA + CO2 + reduced acceptor | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GCD | - |
Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.5 | - |
glutaconyl-CoA | - |
Homo sapiens | |
5.6 | - |
glutaryl-CoA | at pH 6.5 | Homo sapiens | |
11.3 | - |
glutaryl-CoA | at pH 7.6 | Homo sapiens | |
13.2 | - |
glutaryl-CoA | at pH 8.5 | Homo sapiens |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8.5 | - |
Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | oxidation of reduced FAD is much faster than the chemical steps that occur in the active site of the enzyme | Homo sapiens |