KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.003 | 0.005 | glutaryl-CoA | - |
Pseudomonas sp. | |
0.006 | 0.011 | glutaconyl-CoA | - |
Pseudomonas sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
41000 | - |
4 * 41000, SDS-PAGE | Pseudomonas sp. |
170000 | - |
gel filtration | Pseudomonas sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
strains KB 740, K 172 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, 20 mM potassium phosphate, 150 mM NaCl, pH 6.4, several months without loss of activity | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutaconyl-CoA + acceptor | - |
Pseudomonas sp. | crotonyl-CoA + CO2 + reduced acceptor | - |
r | |
glutaryl-CoA + electron-transfer flavoprotein | - |
Pseudomonas sp. | crotonyl-CoA + CO2 + reduced electron-transfer flavoprotein | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 41000, SDS-PAGE | Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | 8 | - |
Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | 1.1 mol per mol of subunit | Pseudomonas sp. |