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Literature summary for 1.3.8.6 extracted from
- Partial purification and characterization of glutaryl-coenzyme A dehydrogenase, electron transfer flavoprotein, and electron transfer flavoprotein-Q oxidoreductase from Paracoccus denitrificans (1985), J. Bacteriol., 163, 709-715.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acetoacetyl-CoA | - |
Paracoccus denitrificans |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0011 | 0.0025 | electron-transfer flavoprotein | - |
Paracoccus denitrificans | |
| 0.0012 | 0.01 | glutaryl-CoA | - |
Paracoccus denitrificans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43000 | - |
4 * 43000, SDS-PAGE | Paracoccus denitrificans |
| 180000 | - |
gel filtration | Paracoccus denitrificans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Paracoccus denitrificans | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Paracoccus denitrificans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| glutaryl-CoA + acceptor | acceptors: electron-transfer flavoprotein, phenylmethylsulfonyl fluoride, 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue | Paracoccus denitrificans | crotonyl-CoA + CO2 + reduced acceptor | - |
? | |
| glutaryl-CoA + electron-transfer flavoprotein | - |
Paracoccus denitrificans | crotonyl-CoA + CO2 + reduced electron-transfer flavoprotein | - |
r | |
| glutaryl-CoA + phenylmethylsulfonyl fluoride | - |
Paracoccus denitrificans | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 43000, SDS-PAGE | Paracoccus denitrificans |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 16 | - |
phenylmethylsulfonyl fluoride | - |
Paracoccus denitrificans | |
| 16 | - |
electron-transfer flavoprotein | - |
Paracoccus denitrificans | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | 8.5 | glutaryl-CoA + electron-transfer flavoprotein | Paracoccus denitrificans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
rapid loss of activity at pH over 7 | Paracoccus denitrificans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Paracoccus denitrificans | |
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