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Literature summary for 1.3.8.5 extracted from

  • Ikeda, Y.; Tanaka, K.
    2-Methyl branched-chain acyl-CoA dehydrogenase from rat liver (1988), Methods Enzymol., 166, 360-373.
    View publication on PubMed
Search for more literature for 1.3.8.5

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 0.1 mM, complete inhibition Rattus norvegicus
Cu2+ 0.1 mM, complete inhibition Rattus norvegicus
Hg2+ 0.1 mM, complete inhibition Rattus norvegicus
Methylmercury iodide 0.1 mM, 50% inhibition Rattus norvegicus
N-ethylmaleimide 2 mM, 70% inhibition Rattus norvegicus
p-hydroxymercuribenzoate 0.1 mM, severe inhibition Rattus norvegicus
tiglyl-CoA competitive vs. (S)-2-methylbutyryl-CoA and isobutyryl-CoA Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
 2-methylbutyryl-CoA
-
 Rattus norvegicus
0.089
-
 isobutyryl-CoA
-
 Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41500
-
 4 * 41500, SDS-PAGE Rattus norvegicus
170000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-methylbutanoyl-CoA + electron transfer flavoprotein Rattus norvegicus enzyme catalyzes the third step in the oxidative metabolism of isoleucin and valine 2-methylbut-2-enoyl-CoA + reduced electron transfer flavoprotein
-
 r

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Rattus norvegicus

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.345
-
 isobutyryl-CoA as substrate Rattus norvegicus
2.476
-
 (S)-2-methylbutyryl-CoA as substrate Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-2-methylbutyryl-CoA + acceptor 22% of activity with (S)-2-methylbutyryl-CoA Rattus norvegicus 2-ethylprop-2-enoyl-CoA + reduced acceptor
-
 ?
(S)-2-methylbutanoyl-CoA + acceptor acceptors: electron transfer flavoprotein, phenazine methosulfate, meldola's blue Rattus norvegicus 2-methylbut-2-enoyl-CoA + reduced acceptor
-
 r
2-methylbutanoyl-CoA + electron transfer flavoprotein enzyme catalyzes the third step in the oxidative metabolism of isoleucin and valine Rattus norvegicus 2-methylbut-2-enoyl-CoA + reduced electron transfer flavoprotein
-
 r
isobutyryl-CoA + acceptor
-
 Rattus norvegicus methacrylyl-CoA + reduced acceptor
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 41500, SDS-PAGE Rattus norvegicus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
 2-methylbutyryl-CoA or isobutyryl-CoA + phenazine methosulfate Rattus norvegicus

Cofactor

Cofactor Comment Organism Structure
FAD
-
 Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.003
-
 2-methylbut-2-enoyl-CoA isobutyryl-CoA as substrate Rattus norvegicus
0.007
-
 2-methylbut-2-enoyl-CoA (S)-2-methylbutyryl-CoA as substrate Rattus norvegicus