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Literature summary for 1.3.8.4 extracted from
- Purification, characterization and cloning of isovaleryl-CoA dehydrogenase from higher plant mitochondria (2001), Eur. J. Biochem., 268, 1332-1339.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| clone obtained from the screening of an Arabidopsis whole plant library cDNA library | Arabidopsis thaliana |
| cloning of cDNAs of two distinct potato enzyme genes, clone obtained from the screening of a potato flower cDNA library | Solanum tuberosum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.047 | - |
isovaleryl-CoA | - |
Solanum tuberosum |  |
| 0.79 | - |
isobutyryl-CoA | - |
Solanum tuberosum | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Solanum tuberosum | 5739 | - |
| mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 75000 | 85000 | gel filtration | Solanum tuberosum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| isovaleryl-CoA + acceptor | Solanum tuberosum | - |
3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
| isovaleryl-CoA + acceptor | Arabidopsis thaliana | - |
3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
| Solanum tuberosum | - |
potato | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| using chromatography on Resource Q anion exchange column, BioRad CHT2 hydroxyapatite column and phenyl ether hydrophobic interaction column | Solanum tuberosum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| tuber | - |
Solanum tuberosum | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1 | - |
substrate: isovaleryl-CoA | Solanum tuberosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-methylpalmitoyl-CoA + acceptor | minor activity | Solanum tuberosum | 2-methylhexadec-2-enoyl-CoA + reduced acceptor | - |
? | |
| 2-methylpalmitoyl-CoA + acceptor | minor activity | Arabidopsis thaliana | 2-methylhexadec-2-enoyl-CoA + reduced acceptor | - |
? | |
| isobutyryl-CoA + acceptor | the activity with isobutyryl-CoA implies an additional role of the enzyme in the catabolism of valine | Solanum tuberosum | 2-methylpropanoyl-CoA + reduced acceptor | - |
? | |
| isobutyryl-CoA + acceptor | the activity with isobutyryl-CoA implies an additional role of the enzyme in the catabolism of valine | Arabidopsis thaliana | 2-methylpropanoyl-CoA + reduced acceptor | - |
? | |
| isovaleryl-CoA + acceptor | - |
Solanum tuberosum | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
| isovaleryl-CoA + acceptor | - |
Arabidopsis thaliana | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
| isovaleryl-CoA + acceptor | isovaleryl-CoA is the preferred substrate | Solanum tuberosum | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
| isovaleryl-CoA + acceptor | isovaleryl-CoA is the preferred substrate | Arabidopsis thaliana | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
| isovaleryl-CoA + acceptor | electron acceptor: 2,6-dichloroindophenol, intermediate electron carrier: phenazine methosulfate | Solanum tuberosum | 3-methylcrotonyl-CoA + reduced acceptor | - |
? | |
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