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Literature summary for 1.3.8.4 extracted from

  • Rhead, W.J.; Hall, C.; Tanaka, K.
    Novel tritium release assays for isovaleryl-CoA and butyryl-CoA dehydrogenases (1981), J. Biol. Chem., 256, 1616-1624.
    View publication on PubMed
Search for more literature for 1.3.8.4

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0063
-
 isovaleryl-CoA sonic supernatant Rattus norvegicus
0.0113
-
 isovaleryl-CoA
-
 Cavia porcellus
0.0113
-
 isovaleryl-CoA purified enzyme in the absence of electron transfer agents Rattus norvegicus
0.013
-
 isovaleryl-CoA whole sonicate Rattus norvegicus
0.0132
-
 isovaleryl-CoA
-
 Cavia porcellus
0.0132
-
 isovaleryl-CoA purified enzyme with electron-transferring flavoprotein and dichlorophenol indophenol, reaction terminated with KMnO4/HCl Rattus norvegicus
0.0208
-
 isovaleryl-CoA
-
 Cavia porcellus
0.0208
-
 isovaleryl-CoA purified enzyme with phenazine methosulfate and dichlorophenol indophenol, reaction terminated with KMnO4/HCl Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
isovaleryl-CoA + acceptor Cavia porcellus
-
 3-methylcrotonyl-CoA + reduced acceptor
-
 ?
isovaleryl-CoA + acceptor Rattus norvegicus
-
 3-methylcrotonyl-CoA + reduced acceptor
-
 ?

Organism

Organism UniProt Comment Textmining
Cavia porcellus
-
-
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
 Cavia porcellus
-
liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.00003
-
 intact mitochondria, no added acceptor Rattus norvegicus
0.00016
-
 sonicated mitochondria, no added acceptor Rattus norvegicus
0.00044
-
 sonicated mitochondria, acceptor added: electron-transferring flavoprotein Rattus norvegicus
0.00046
-
 sonicated mitochondria, acceptor added: phenazine methosulfate Rattus norvegicus
0.0062
-
 purified enzyme in the absence of electron transfer agents, reaction terminated with HCl or with KMnO4/HCl Cavia porcellus
0.0136
-
 purified enzyme with phenazine methosulfate and dichlorophenol indophenol, reaction terminated with HCl Cavia porcellus
0.0207
-
 purified enzyme with electron-transferring flavoprotein and 0.062 mM dichlorophenol indophenol, reaction terminated with HCl, concentration of isovaleryl-CoA: 0.05 mM, specific activity determined by tritium release assay Cavia porcellus
0.0337
-
 purified enzyme, acceptor added: electron-transferring flavoprotein Cavia porcellus
0.043
-
 purified enzyme, acceptor added: electron-transferring flavoprotein and 0.062 mM dichlorophenol indophenol, reaction terminated with KMnO4/HCl, concentration of isovaleryl-CoA: 0.02 mM, specific activity determined by tritium release assay Cavia porcellus
0.043
-
 purified enzyme, acceptor added: phenazine methosulfate Cavia porcellus
0.0472
-
 purified enzyme with phenazine methosulfate and dichlorophenol indophenol, reaction terminated with KMnO4/HCl Cavia porcellus
0.0572
-
 purified enzyme with electron-transferring flavoprotein and 0.062 mM dichlorophenol indophenol, reaction terminated with KMnO4/HCl, concentration of isovaleryl-CoA: 0.05 mM, specific activity determined by tritium release assay Cavia porcellus
0.172
-
 purified enzyme, acceptor added: electron-transferring flavoprotein and 0.062 mM dichlorophenol indophenol, concentration of isovaleryl-CoA: 0.02 mM, specific activity determined by dye reduction assay Cavia porcellus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
isovaleryl-CoA + acceptor
-
 Cavia porcellus 3-methylcrotonyl-CoA + reduced acceptor
-
 ?
isovaleryl-CoA + acceptor
-
 Rattus norvegicus 3-methylcrotonyl-CoA + reduced acceptor
-
 ?
isovaleryl-CoA + acceptor enzyme activity observed in the absence of electron transfer agents suggests that rapid tritium exchange occurs between the enzyme-bound substrate and the incubation medium Cavia porcellus 3-methylcrotonyl-CoA + reduced acceptor
-
 ?
isovaleryl-CoA + acceptor acceptors: electron-transfer flavoprotein, phenazine methosulfate Cavia porcellus 3-methylcrotonyl-CoA + reduced acceptor
-
 ?
isovaleryl-CoA + acceptor acceptors: electron-transfer flavoprotein, phenazine methosulfate Rattus norvegicus 3-methylcrotonyl-CoA + reduced acceptor
-
 ?