KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.014 | - |
butyryl-CoA | - |
Bos taurus | |
0.083 | - |
ferricytochrome c | - |
Bos taurus | |
0.33 | - |
2,6-dichlorophenolindophenol | - |
Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
120000 | 220000 | analytical ultracentrifugation | Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Bos taurus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
DELTA D600 = 12.2/min/mg protein | Bos taurus |
0.77 | - |
- |
Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
butyryl-CoA + electron acceptor | - |
Bos taurus | 2-butenoyl-CoA + reduced acceptor | - |
r | |
butyryl-CoA + ferricytochrome c | - |
Bos taurus | butenoyl-CoA + reduced acceptor | - |
r | |
butyryl-CoA + pyocyanine + triphenyltetrazolium chloride | - |
Bos taurus | 2-butenoyl-CoA + reduced acceptor | - |
r | |
hexanoyl-CoA + phenazine methosulfate + dichloroindophenol | 40% of activity with butyryl-CoA | Bos taurus | 2-hexenoyl-CoA + reduced acceptor | - |
? | |
pentanoyl-CoA + 2,6-dichlorophenolindophenol + phenazine ethosulfate | - |
Bos taurus | 2-pentenoyl-CoA + reduced acceptor | - |
? | |
propionyl-CoA + 2,6-dichlorophenolindophenol | 30% of activity with butyryl-CoA | Bos taurus | 2-propenoyl-CoA + reduced acceptor | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
33.3 | - |
butyryl-CoA | based on an estimated MW of 150000 Da | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Bos taurus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 8 | 42% activity at pH 6.5, 78% at pH 7.5 and 65% at pH 8.0 | Bos taurus |