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Literature summary for 1.3.7.8 extracted from

  • Boll, M.; Fuchs, G.
    Benzoyl-coenzyme A reductase (dearomatizing), a key enzyme of anaerobic aromatic metabolism. ATP dependence of the reaction, purification and some properties of the enzyme from Thaurea aromatica strain K172 (1995), Eur. J. Biochem., 234, 921-933.
    View publication on PubMed

General Stability

General Stability Organism
oxygen-sensitive Thauera aromatica

Inhibitors

Inhibitors Comment Organism Structure
2,2'-bipyridyl
-
Thauera aromatica
Acetylene
-
Thauera aromatica
ATP above 12 mM Thauera aromatica
ethylene
-
Thauera aromatica

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
benzoyl-CoA
-
Thauera aromatica
0.15
-
NH2OH
-
Thauera aromatica
0.6
-
ATP
-
Thauera aromatica

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 15% of the activity with Mg2+ Thauera aromatica
Fe2+ 35% of the activity with Mg2+ Thauera aromatica
Iron contains 10.8 mol Fe and 10.5 mol acid-labile sulfur per mol of enzyme Thauera aromatica
Mg2+ specific for MgATP2- Thauera aromatica
Mn2+ 52% of the activity with Mg2+ Thauera aromatica
Zinc contains 0.5 mol of Zn per mol of enzyme Thauera aromatica

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
32000
-
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE Thauera aromatica
38000
-
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE Thauera aromatica
45000
-
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE Thauera aromatica
48000
-
1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE Thauera aromatica
170000
-
gel filtration Thauera aromatica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
Benzoyl-CoA + reduced acceptor + ATP Thauera aromatica key enzyme of anaerobic aromatic metabolism ?
-
?

Organism

Organism UniProt Comment Textmining
Thauera aromatica
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thauera aromatica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.55
-
-
Thauera aromatica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-aminobenzoyl-CoA + reduced methyl viologen + ATP at 18% of the activity relative to benzoyl-CoA Thauera aromatica ?
-
?
2-fluorobenzoyl-CoA + reduced methyl viologen + ATP at 78% of the activity relative to benzoyl-CoA Thauera aromatica ?
-
?
2-fluorobenzoyl-CoA + reduced methyl viologen + ATP at 10% of the activity relative to benzoyl-CoA Thauera aromatica ?
-
?
3-fluorobenzoyl-CoA + reduced methyl viologen + ATP at 23% of the activity relative to benzoyl-CoA Thauera aromatica ?
-
?
3-hydroxybenzoyl-CoA + reduced methyl viologen + ATP at 12% of the activity relative to benzoyl-CoA Thauera aromatica ?
-
?
3-methylbenzoyl-CoA + reduced methyl viologen + ATP at 12% of the activity relative to benzoyl-CoA Thauera aromatica ?
-
?
4-hydroxybenzoyl-CoA + reduced methyl viologen + ATP at 5% of the activity relative to benzoyl-CoA Thauera aromatica 4-hydroxycyclohexa-1,5-diene-1-carbonyl-CoA + oxidized methyl viologen + ADP + phosphate
-
?
azide + ATP + H2O
-
Thauera aromatica ?
-
?
benzoyl-CoA + reduced acceptor + ATP acceptor: Ti3+, reduced methyl viologen Thauera aromatica cyclohexa-1,5-diene-1-carbonyl-CoA + acceptor + ADP + phosphate
-
?
Benzoyl-CoA + reduced acceptor + ATP key enzyme of anaerobic aromatic metabolism Thauera aromatica ?
-
?
additional information in absence of benzoyl-CoA the enzyme exhibits oxygen-sensitive ATPase activity Thauera aromatica ?
-
?
N-methylhydroxylamine + ATP + H2O
-
Thauera aromatica ?
-
?
NH2OH + ATP + H2O
-
Thauera aromatica ?
-
?
O-methylhydroxylamine + ATP + H2O
-
Thauera aromatica ?
-
?

Subunits

Subunits Comment Organism
tetramer 1 * 32000 + 1 * 38000 + 1 * 45000 + 1 * 48000, SDS-PAGE Thauera aromatica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2 7.5
-
Thauera aromatica

pH Range

pH Minimum pH Maximum Comment Organism
6.6 8.2 50% of maximal activity at pH 6.6 and 8.2, less than 10% of maximal activity at pH 6.2 and 8.7 Thauera aromatica

Cofactor

Cofactor Comment Organism Structure
flavin contains 0.3 mol flavin or flavin-like compound per mol of enzyme Thauera aromatica