Literature summary for 1.3.7.5 extracted from

Alvey, R.M.; Biswas, A.; Schluchter, W.M.; Bryant, D.A.
Attachment of noncognate chromophores to CpcA of Synechocystis sp. PCC 6803 and Synechococcus sp. PCC 7002 by heterologous expression in Escherichia coli (2011), Biochemistry, 50, 4890-4902.

Cloned(Commentary)

Cloned (Comment)	Organism
recombinant functional expression of PcyA in Escherichia coli with production of phycocyanobilin, functional co-expression with cyanobacterial heme oxygenase, and the phycocyanin alpha-subunit, CpcA, from Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002, and with the phycocyanin alpha-subunit phycocyanobilin lyase, CpcE/CpcF, or the phycoerythrocyanin alpha-subunit phycocyanobilin isomerizing lyase, PecE/PecF, from Noctoc sp. PCC 7120. Production levels of fluorescent pigments and chromophore analysis, overview	Synechococcus sp.

Cloned (Comment)

Organism

recombinant functional expression of PcyA in Escherichia coli with production of phycocyanobilin, functional co-expression with cyanobacterial heme oxygenase, and the phycocyanin alpha-subunit, CpcA, from Synechocystis sp. PCC 6803 or Synechococcus sp. PCC 7002, and with the phycocyanin alpha-subunit phycocyanobilin lyase, CpcE/CpcF, or the phycoerythrocyanin alpha-subunit phycocyanobilin isomerizing lyase, PecE/PecF, from Noctoc sp. PCC 7120. Production levels of fluorescent pigments and chromophore analysis, overview

Synechococcus sp.

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
biliverdin Ixalpha + reduced ferredoxin	Synechococcus sp.	-	(3Z)-phycocyanobilin + oxidized ferredoxin	-	?
biliverdin Ixalpha + reduced ferredoxin	Synechococcus sp. 7002	-	(3Z)-phycocyanobilin + oxidized ferredoxin	-	?

Organism

Organism	UniProt	Comment	Textmining
Synechococcus sp.	-	-	-
Synechococcus sp. 7002	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
biliverdin Ixalpha + reduced ferredoxin	-	Synechococcus sp.	(3Z)-phycocyanobilin + oxidized ferredoxin	-	?
biliverdin Ixalpha + reduced ferredoxin	-	Synechococcus sp. 7002	(3Z)-phycocyanobilin + oxidized ferredoxin	-	?

Synonyms

Synonyms	Comment	Organism
3Z-phycocyanobilin:ferredoxin oxidoreductase	-	Synechococcus sp.
PcyA	-	Synechococcus sp.

Cofactor

Cofactor	Comment	Organism	Structure
Ferredoxin	-	Synechococcus sp.

General Information

General Information	Comment	Organism
additional information	cyanobacteria utilize phycocyanobilin:ferredoxin oxidoreductase (PcyA) to perform a two-step reaction, the enzyme first reduces the 18-vinyl side chain of the D-ring and subsequently reduces the vinyl side chain of the pyrrole A ring to yield phycocyanobilin	Synechococcus sp.