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Literature summary for 1.3.7.5 extracted from
- HO1 and PcyA proteins involved in phycobilin biosynthesis form a 1:2 complex with ferredoxin-1 required for photosynthesis (2009), FEBS Lett., 583, 1251-1256.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| PcyA expression in Escherichia coli strain BL21(DE3) | Thermosynechococcus vestitus |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 27000 | - |
x * 27000, recombinant enzyme, SDS-PAGE | Thermosynechococcus vestitus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| biliverdin Ixalpha + reduced ferredoxin | Thermosynechococcus vestitus | - |
(3Z)-phycocyanobilin + oxidized ferredoxin | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermosynechococcus vestitus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli strain BL21(DE3) by anion-exchange chromatography, hydrophobic interaction chromatography, hydroxyapatite chromatography, and gel filtration | Thermosynechococcus vestitus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -30°C, purified recombinant enzyme, 50 mM HEPES-NaOH, pH 7.5, containing 100 mM NaCl, 6 months, full activity is maintained | Thermosynechococcus vestitus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| biliverdin Ixalpha + reduced ferredoxin | - |
Thermosynechococcus vestitus | (3Z)-phycocyanobilin + oxidized ferredoxin | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 27000, recombinant enzyme, SDS-PAGE | Thermosynechococcus vestitus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Pcb:Fd oxidoreductase | - |
Thermosynechococcus vestitus |
| PcyA | - |
Thermosynechococcus vestitus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Thermosynechococcus vestitus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Thermosynechococcus vestitus |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | PcyA is involved in the phycocyanobilin biosynthetic pathway, in which PcyA first forms a stable complexes with the biliverdin IXa molecule | Thermosynechococcus vestitus |
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Release 2023.1 (January 2023)
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