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Literature summary for 1.3.7.5 extracted from

  • Tu, S.L.; Rockwell, N.C.; Lagarias, J.C.; Fisher, A.J.
    Insight into the radical mechanism of phycocyanobilin-ferredoxin oxidoreductase (PcyA) revealed by X-ray crystallography and biochemical measurements (2007), Biochemistry, 46, 1484-1494.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapour diffusion method with 18% PEG-8000, 0.025 M MES-NaOH at pH 6.5, 0.05 M Ca(OAc)2 at pH 6.5, and 5% dioxane (or 5% ethanol) Nostoc sp. PCC 7120 = FACHB-418

Protein Variants

Protein Variants Comment Organism
D102N exhibits 11% of wild type activity Nostoc sp. PCC 7120 = FACHB-418
E73Q exhibits 20% of wild type activity Nostoc sp. PCC 7120 = FACHB-418
H71Q exhibits 65% of wild type activity Nostoc sp. PCC 7120 = FACHB-418
H85Q exhibits 5% of wild type activity Nostoc sp. PCC 7120 = FACHB-418
K218E exhibits 20% of wild type activity Nostoc sp. PCC 7120 = FACHB-418

Organism

Organism UniProt Comment Textmining
Nostoc sp. PCC 7120 = FACHB-418 Q93TN0 also known as Anabaena sp. strain PCC7120
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Purification (Commentary)

Purification (Comment) Organism
Superdex G-200 gel filtration Nostoc sp. PCC 7120 = FACHB-418

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
biliverdin IXalpha + reduced ferredoxin
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Nostoc sp. PCC 7120 = FACHB-418 phycocyanobilin + oxidized ferredoxin
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?

Synonyms

Synonyms Comment Organism
PcyA
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Nostoc sp. PCC 7120 = FACHB-418
phycocyanobilin-ferredoxin oxidoreductase
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Nostoc sp. PCC 7120 = FACHB-418