BRENDA - Enzyme Database show
show all sequences of 1.3.7.3

Metabolic engineering to produce phytochromes with phytochromobilin, phycocyanobilin, or phycoerythrobilin chromophore in Escherichia coli

Mukougawa, K.; Kanamoto, H.; Kobayashi, T.; Yokota, A.; Kohchi, T.; FEBS Lett. 580, 1333-1338 (2006)

Data extracted from this reference:

Application
Application
Commentary
Organism
additional information
metabolic engineering of bacteria for the production of various bilins for assembly into phytochromes will facilitate the molecular analysis of photoreceptors
Synechococcus sp.
Cloned(Commentary)
Commentary
Organism
into vector pKT270, co-expression with cyanobacterial phytochrome 1 in Escherichia coli JM109
Synechococcus sp.
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Synechococcus sp.
-
-
-
Synechococcus sp. W8020
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
15,16-dihydrobiliverdin + reduced ferredoxin
-
673636
Synechococcus sp.
(3Z)-phycoerythrobilin + oxidized ferredoxin
-
-
-
?
15,16-dihydrobiliverdin + reduced ferredoxin
-
673636
Synechococcus sp. W8020
(3Z)-phycoerythrobilin + oxidized ferredoxin
-
-
-
?
Application (protein specific)
Application
Commentary
Organism
additional information
metabolic engineering of bacteria for the production of various bilins for assembly into phytochromes will facilitate the molecular analysis of photoreceptors
Synechococcus sp.
Cloned(Commentary) (protein specific)
Commentary
Organism
into vector pKT270, co-expression with cyanobacterial phytochrome 1 in Escherichia coli JM109
Synechococcus sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
15,16-dihydrobiliverdin + reduced ferredoxin
-
673636
Synechococcus sp.
(3Z)-phycoerythrobilin + oxidized ferredoxin
-
-
-
?
15,16-dihydrobiliverdin + reduced ferredoxin
-
673636
Synechococcus sp. W8020
(3Z)-phycoerythrobilin + oxidized ferredoxin
-
-
-
?
Other publictions for EC 1.3.7.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742861
Overkamp
Insights into the biosynthesi ...
Guillardia theta
J. Biol. Chem.
289
26691-26707
2014
-
-
1
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-
-
-
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5
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1
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2
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1
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2
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-
-
-
724228
Busch
Structural and mechanistic ins ...
Synechococcus sp., Synechococcus sp. WH8020
Biochem. J.
439
257-264
2011
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1
-
4
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2
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4
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2
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1
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1
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1
1
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4
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2
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2
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1
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3
3
-
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-
673636
Mukougawa
Metabolic engineering to produ ...
Synechococcus sp., Synechococcus sp. W8020
FEBS Lett.
580
1333-1338
2006
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1
1
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2
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674639
Dammeyer
Insights into phycoerythrobili ...
Microchaete diplosiphon, Microchaete diplosiphon Fd33, Synechococcus sp., Synechococcus sp. WH8020
J. Biol. Chem.
281
27081-27089
2006
-
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2
-
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-
-
-
-
-
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13
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2
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4
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4
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2
4
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2
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4
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656968
Alvey
Lesions in phycoerythrin chrom ...
Microchaete diplosiphon
Plant Cell
15
2448-2463
2003
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-
-
-
-
-
-
-
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1
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1
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1
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1
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437673
Frankenberg
Functional genomic analysis of ...
Nostoc punctiforme, Prochlorococcus sp., Synechococcus sp.
Plant Cell
13
965-978
2001
-
-
3
-
-
-
-
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1
3
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3
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3
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6
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2
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3
2
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1
3
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3
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6
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