KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | oligomeric BciA provides a kcat/Km value of 0.029/s * microM, which is 15fold higher than the value of a-COR towards Chlide a (0.0019/s * microM when the ratio of BchX:BchYZ is 4:1) | Rhodobacter capsulatus | |
0.0059 | - |
8-vinyl chlorophyllide a | pH 8.0, 22°C, recombinant BchYZ heterodimer | Rhodobacter capsulatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | Rhodobacter capsulatus | - |
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | Rhodobacter capsulatus NBRC 16581 | - |
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | Rhodobacter capsulatus ATCC BAA-309 | - |
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | Rhodobacter capsulatus SB1003 | - |
bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | Rhodobacter capsulatus | - |
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | Rhodobacter capsulatus NBRC 16581 | - |
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | Rhodobacter capsulatus ATCC BAA-309 | - |
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | Rhodobacter capsulatus SB1003 | - |
chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodobacter capsulatus | P26177 AND P26178 AND P26179 | genes bchX, bchY, and bchZ encoding the three subunits of the enzyme | - |
Rhodobacter capsulatus ATCC BAA-309 | P26177 AND P26178 AND P26179 | genes bchX, bchY, and bchZ encoding the three subunits of the enzyme | - |
Rhodobacter capsulatus NBRC 16581 | P26177 AND P26178 AND P26179 | genes bchX, bchY, and bchZ encoding the three subunits of the enzyme | - |
Rhodobacter capsulatus SB1003 | P26177 AND P26178 AND P26179 | genes bchX, bchY, and bchZ encoding the three subunits of the enzyme | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | - |
Rhodobacter capsulatus | bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | - |
Rhodobacter capsulatus NBRC 16581 | bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | - |
Rhodobacter capsulatus ATCC BAA-309 | bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
3-acetyl-3-devinylchlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + ATP + H2O + 2 H+ | - |
Rhodobacter capsulatus SB1003 | bacteriochlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster + ADP + phosphate | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Rhodobacter capsulatus | chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Rhodobacter capsulatus NBRC 16581 | chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Rhodobacter capsulatus ATCC BAA-309 | chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? | |
8-vinyl chlorophyllide a + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ | - |
Rhodobacter capsulatus SB1003 | chlorophyllide a + 2 oxidized ferredoxin [iron-sulfur] cluster | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | one BchYZ heterodimer is assumed as a minimal active protomer | Rhodobacter capsulatus |
Synonyms | Comment | Organism |
---|---|---|
a-COR | - |
Rhodobacter capsulatus |
BchX | - |
Rhodobacter capsulatus |
BchY | - |
Rhodobacter capsulatus |
BchZ | - |
Rhodobacter capsulatus |
COR | - |
Rhodobacter capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Rhodobacter capsulatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.011 | - |
8-vinyl chlorophyllide a | pH 8.0, 22°C, recombinant BchYZ heterodimer | Rhodobacter capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rhodobacter capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Rhodobacter capsulatus | |
Fe-S center | - |
Rhodobacter capsulatus | |
Ferredoxin | - |
Rhodobacter capsulatus |
General Information | Comment | Organism |
---|---|---|
evolution | BciA (EC 1.3.1.75, NADPH-dependent C8 divinyl reductase) is a much faster and less energy consuming catalytic enzyme than the chlorophyllide oxidoreductase (COR). This finding implies that the bciA gene was acquired in each ancestral lineage by multiple horizontal gene transfer events after the establishment of anoxygenic photosynthesis. The high demand of Chl for large antenna complexes might have a major selective pressure for the evolutionary acquisition of an auxiliary divinyl reductase, BciA. Compared to the enzymatic activity of a-COR, BciA from Rhodobacter capsulatus has a lower affinity for DV-Chlide a but a much higher specific activity | Rhodobacter capsulatus |
physiological function | chlorophyllide (Chlide) oxidoreductase, COR, is a unique BChl biosynthesis enzyme which shows distinct substrate recognition and hydrogen addition activities, depending on the host strain. COR in BChl a-producing phototrophic bacteria (a-COR) catalyzes the C7=C8 double bond reduction of Chlide a, whereas COR in BChl b- and BChl g-producing bacteria (b/g-COR) forms the C8-ethylidene group using DV-Chlide a as the substrate. Chlorophyllide oxidoreductase (COR) reduces the C7=C8 double bond of chlorophyllide a (Chlide a) bearing the C8-ethyl group, but also potentially catalyzes the reduction of the C8-vinyl group of divinyl-chlorophyllide a (DV-Chlide a). DV-Chlide a is a universal precursor for chlorophyll or bacteriochlorophyll biosynthesis in all photosynthetic organisms | Rhodobacter capsulatus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.864 | - |
8-vinyl chlorophyllide a | pH 8.0, 22°C, recombinant BchYZ heterodimer | Rhodobacter capsulatus |