Cloned (Comment) | Organism |
---|---|
crtI gene, construction of different mutation libraries used for screening for mutants which synthesize almost exclusively either neurosporene or lycopene, cloning n Escherichia coli strain DH5alpha | Rubrivivax gelatinosus |
Protein Variants | Comment | Organism |
---|---|---|
D355G | naturally occuring mutation | Rubrivivax gelatinosus |
D53G | naturally occuring mutation | Rubrivivax gelatinosus |
L153P | naturally occuring mutation, which influences the secondary structure of the enzyme | Rubrivivax gelatinosus |
L208F | naturally occuring mutation, which influences the secondary structure of the enzyme | Rubrivivax gelatinosus |
L208P | naturally occuring mutation, which influences the secondary structure of the enzyme | Rubrivivax gelatinosus |
L278P | naturally occuring mutation, which influences the secondary structure of the enzyme | Rubrivivax gelatinosus |
L424P | naturally occuring mutation, which influences the secondary structure of the enzyme | Rubrivivax gelatinosus |
additional information | in mutant EP21, zeta-carotene and phytofluene, which are both early intermediates of the desaturation pathway and which are absent in the wild-type, are the main carotene products | Rubrivivax gelatinosus |
P134L | naturally occuring mutation, which influences the secondary structure of the enzyme | Rubrivivax gelatinosus |
T256M | naturally occuring mutation | Rubrivivax gelatinosus |
V395A | naturally occuring mutation | Rubrivivax gelatinosus |
Y44C | naturally occuring mutation | Rubrivivax gelatinosus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0048 | - |
phytoene | pH 8.0, 28°C, recombinant mutant L208F | Rubrivivax gelatinosus | |
0.0148 | - |
phytoene | pH 8.0, 28°C, recombinant wild-type enzyme | Rubrivivax gelatinosus | |
0.0171 | - |
phytoene | pH 8.0, 28°C, recombinant mutant L208P | Rubrivivax gelatinosus | |
0.0331 | - |
7,9,9'-tricis-neurosporene | pH 8.0, 28°C, recombinant wild-type enzyme | Rubrivivax gelatinosus | |
0.0417 | - |
7,9,9'-tricis-neurosporene | pH 8.0, 28°C, recombinant mutant L208P | Rubrivivax gelatinosus | |
0.0658 | - |
7,9,9'-tricis-neurosporene | pH 8.0, 28°C, recombinant mutant L208F | Rubrivivax gelatinosus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | transmembrane enzyme, model, overview | Rubrivivax gelatinosus | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,9,9'-tricis-neurosporene + a quinone | Rubrivivax gelatinosus | - |
7,9,7',9'-tetracis-lycopene + a quinol | - |
? | |
7,9,9'-tricis-neurosporene + a quinone | Rubrivivax gelatinosus CGMCC 6086 | - |
7,9,7',9'-tetracis-lycopene + a quinol | - |
? | |
phytoene + quinone | Rubrivivax gelatinosus | - |
7,9,9'-tricis-neurosporene + quinol | - |
? | |
phytoene + quinone | Rubrivivax gelatinosus CGMCC 6086 | - |
7,9,9'-tricis-neurosporene + quinol | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rubrivivax gelatinosus | - |
gene crtI | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
7,9,9'-tricis-neurosporene + a quinone | - |
Rubrivivax gelatinosus | 7,9,7',9'-tetracis-lycopene + a quinol | - |
? | |
7,9,9'-tricis-neurosporene + a quinone | - |
Rubrivivax gelatinosus CGMCC 6086 | 7,9,7',9'-tetracis-lycopene + a quinol | - |
? | |
phytoene + quinone | - |
Rubrivivax gelatinosus | 7,9,9'-tricis-neurosporene + quinol | - |
? | |
phytoene + quinone | - |
Rubrivivax gelatinosus CGMCC 6086 | 7,9,9'-tricis-neurosporene + quinol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CrtI | - |
Rubrivivax gelatinosus |
phytoene desaturase | - |
Rubrivivax gelatinosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
28 | - |
assay at | Rubrivivax gelatinosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Rubrivivax gelatinosus |
General Information | Comment | Organism |
---|---|---|
metabolism | phytoene desaturase CrtI from Rubrivivax gelatinosus catalyzes simultaneously a three- and four-step desaturation producing both neurosporene and lycopene. These carotenes are intermediates for the synthesis of spheroidene and spirilloxanthin, respectively | Rubrivivax gelatinosus |
additional information | Leu208, located in a highly hydrophobic membrane-integrated region, is important in substrate binding | Rubrivivax gelatinosus |