Literature summary for 1.3.5.2 extracted from

Knecht, W.; Bergjohann, U.; Gonski, S.; Kirschbaum, B.; Loffler, M.
Functional expression of a fragment of human dihydroorotate dehydrogenase by means of the baculovirus expression vector system, and kinetic investigation of the purified recombinant enzyme (1996), Eur. J. Biochem., 240, 292-301.

Search for more literature for 1.3.5.2

Inhibitors

Inhibitors	Comment	Organism	Structure
2-hydroxyethylidene-cyanoacetic acid 4-trifluoromethyl anilide	A77 1726, active metabolite of leflumonide	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.004	-	dihydroorotate	cosubstrate ubiquinone-50	Homo sapiens
0.009	-	dihydroorotate	cosubstrate decylubiquinone	Homo sapiens
0.01	-	ubiquinone-50	cosubstrate dihydroorotate	Homo sapiens
0.014	-	decylubiquinone	cosubstrate dihydroorotate	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
mitochondrion	-	Homo sapiens	5739	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
42000	-	gel filtration, truncated recombinant protein	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	truncated recombinant protein	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-dihydroorotate + ubiquinone-50	-	Homo sapiens	orotate + ubiquinol-50	-	r
dihydroorotate + acceptor	acceptor: ubiquinone	Homo sapiens	orotate + reduced acceptor	-	?
L-dihydroorotate + decylubiquinone	-	Homo sapiens	orotate + reduced decylubiquinone	-	?

Subunits

Subunits	Comment	Organism
monomer	SDS-PAGE, truncated recombinant protein	Homo sapiens

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.0002	0.0009	2-hydroxyethylidene-cyanoacetic acid 4-trifluoromethyl anilide	competitive to dihydroorotate	Homo sapiens
0.001	-	2-hydroxyethylidene-cyanoacetic acid 4-trifluoromethyl anilide	non-competitive to decyclubiquinone	Homo sapiens

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Release 2023.1 (January 2023)