Inhibitors | Comment | Organism | Structure |
---|---|---|---|
papyriferic acid | papyriferic acid is a triterpene that is secreted by glands on twigs of the juvenile ontogenetic phase of resin producing tree birches. Papyriferic acid is a potent inhibitor of SDH. Kinetic analysis indicate that, unlike malonate, papyriferic acid acts by an uncompetitive mechanism, by binding to the enzyme-substrate complex. The hydrolysis product of papyriferic acid, betulafolienetriol oxide, is inactive on SDH. Papyriferic acid acts as an intact molecule and interacts at a site other than the succinate binding site, possibly binding to the ubiquinone sites on complex II | Bos taurus | |
papyriferic acid | papyriferic acid is a triterpene that is secreted by glands on twigs of the juvenile ontogenetic phase of resin producing tree birches. Papyriferic acid is a potent inhibitor of SDH. Kinetic analysis indicate that, unlike malonate, papyriferic acid acts by an uncompetitive mechanism, by binding to the enzyme-substrate complex. The hydrolysis product of papyriferic acid, betulafolienetriol oxide, is inactive on SDH. Papyriferic acid acts as an intact molecule and interacts at a site other than the succinate binding site, possibly binding to the ubiquinone sites on complex II | Oryctolagus sp. | |
papyriferic acid | papyriferic acid is a triterpene that is secreted by glands on twigs of the juvenile ontogenetic phase of resin producing tree birches. Papyriferic acid is a potent inhibitor of SDH. Kinetic analysis indicate that, unlike malonate, papyriferic acid acts by an uncompetitive mechanism, by binding to the enzyme-substrate complex. The hydrolysis product of papyriferic acid, betulafolienetriol oxide, is inactive on SDH. Papyriferic acid acts as an intact molecule and interacts at a site other than the succinate binding site, possibly binding to the ubiquinone sites on complex II | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.138 | - |
succinate | succinate with papyriferic acid 0.04 mM, pH 7.4, 25°C | Oryctolagus sp. | |
0.165 | - |
succinate | succinate with papyriferic acid 0.04 mM, pH 7.4, 25°C | Bos taurus | |
0.167 | - |
succinate | succinate with papyriferic acid 0.08 mM, pH 7.4, 25°C | Rattus norvegicus | |
0.303 | - |
succinate | succinate alone, pH 7.4, 25°C | Bos taurus | |
0.339 | - |
succinate | succinate alone, pH 7.4, 25°C | Oryctolagus sp. | |
0.414 | - |
succinate | succinate alone, pH 7.4, 25°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | the effect of papyriferic acid on the oxidation of succinate by SDH is examined in mitochondrial preparations from livers | Rattus norvegicus | 5739 | - |
mitochondrion | the effect of papyriferic acid on the oxidation of succinate by SDH is examined in mitochondrial preparations from livers | Bos taurus | 5739 | - |
mitochondrion | the effect of papyriferic acid on the oxidation of succinate by SDH is examined in mitochondrial preparations from livers | Oryctolagus sp. | 5739 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Oryctolagus sp. | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
liver | - |
Bos taurus | - |
liver | - |
Oryctolagus sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
succinate + acceptor | succinic acid is incubated with mitochondria and its oxidation by SDH is measured by the reduction of 2,6-dichlorophenol indophenol | Oryctolagus sp. | fumarate + reduced acceptor | - |
? | |
succinate + oxidised 2,6-dichlorophenol indophenol | succinic acid is incubated with mitochondria and its oxidation by SDH is measured by the reduction of 2,6-dichlorophenol indophenol | Rattus norvegicus | fumarate + reduced 2,6-dichlorophenol indophenol | - |
? | |
succinate + oxidised 2,6-dichlorophenol indophenol | succinic acid is incubated with mitochondria and its oxidation by SDH is measured by the reduction of 2,6-dichlorophenol indophenol | Bos taurus | fumarate + reduced 2,6-dichlorophenol indophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
succinate dehydrogenase | - |
Rattus norvegicus |
succinate dehydrogenase | - |
Bos taurus |
succinate dehydrogenase | - |
Oryctolagus sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Rattus norvegicus |
25 | - |
assay at | Bos taurus |
25 | - |
assay at | Oryctolagus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Rattus norvegicus |
7.4 | - |
assay at | Bos taurus |
7.4 | - |
assay at | Oryctolagus sp. |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.02567 | - |
papyriferic acid | pH 7.4, 25°C | Oryctolagus sp. | |
0.03573 | - |
papyriferic acid | pH 7.4, 25°C | Rattus norvegicus | |
0.04527 | - |
papyriferic acid | pH 7.4, 25°C | Bos taurus |