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Literature summary for 1.3.5.1 extracted from
- Purification and characterization of succinate:menaquinone oxidoreductase from Corynebacterium glutamicum (2005), Arch. Microbiol., 183, 317-324.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 2-n-heptyl-4-hydroxyquinoline | inhibition of succinate dehydrogenase activity using 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone or 2,6-dichlorophenolindophenol as electron acceptor | Corynebacterium glutamicum |  |
| 2-n-heptyl-4-hydroxyquinoline N-oxide | inhibitory to the succinate dehydrogenase activity assay using 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone and 2,6-dichloroindophenol as the electron acceptor, not inhibitory to the succinate dehydrogenase activity assay using phenazine methosulfate and 2,6-dichloroindophenol | Corynebacterium glutamicum | |
| p-benzoquinone | inhibition of succinate dehydrogenase activity using phenazone methosulfate or 2,6-dichlorophenolindophenol as electron acceptor; inhibitory to the succinate dehydrogenase activity assay using phenazine methosulfate and 2,6-dichloroindophenol | Corynebacterium glutamicum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.25 | - |
succinate | 30°C, pH 7.0 | Corynebacterium glutamicum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 23000 | - |
3 * 29000 + 3 * 67000 + 3 * 23000, homotrimeric complex of the protomer composed of three different subunits (29000 Da, 67000 Da and 23000 Da), SDS-PAGE | Corynebacterium glutamicum |
| 29000 | - |
3 * 29000 + 3 * 67000 + 3 * 23000, homotrimeric complex of the protomer composed of three different subunits (29000 Da, 67000 Da and 23000 Da), SDS-PAGE | Corynebacterium glutamicum |
| 67000 | - |
3 * 29000 + 3 * 67000 + 3 * 23000, homotrimeric complex of the protomer composed of three different subunits (29000 Da, 67000 Da and 23000 Da), SDS-PAGE | Corynebacterium glutamicum |
| 410000 | - |
PAGE | Corynebacterium glutamicum |
| 410000 | - |
non-denaturing PAGE | Corynebacterium glutamicum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Corynebacterium glutamicum | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Corynebacterium glutamicum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 57.5 | - |
- |
Corynebacterium glutamicum |
| 57.5 | - |
pH 7.0, 30°C | Corynebacterium glutamicum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | efficiency with different quinones, in decreasing order: phenazine methosulfate, decylubiquinone, duroquinone, menadione, 2,3-dimethyl-1,4-naphthoquinone | Corynebacterium glutamicum | ? | - |
? | |
| succinate + 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinone | - |
Corynebacterium glutamicum | fumarate + 2,3-dimethoxy-5-methyl-6-decyl-1,4-benzoquinol | - |
r | |
| succinate + decylubiquinone | - |
Corynebacterium glutamicum | fumarate + ? | - |
? | |
| succinate + phenazine methosulfate | phenazine methosulfate as direct electron acceptor and 2,6-dichlorophenolindophenol as final acceptor | Corynebacterium glutamicum | fumarate + ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | 3 * 29000 + 3 * 67000 + 3 * 23000, homotrimeric complex of the protomer composed of three different subunits (29000 Da, 67000 Da and 23000 Da), SDS-PAGE | Corynebacterium glutamicum |
| trimer | trimer of the heterotrimeric protomer. 67000, subunit SdhA, 29000, subunit SdhB, 23000, subunit SdhC, SDS-PAGE | Corynebacterium glutamicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| SQR | - |
Corynebacterium glutamicum |
| succinate:menaquinone oxidoreductase | - |
Corynebacterium glutamicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
- |
Corynebacterium glutamicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
- |
Corynebacterium glutamicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| heme | enzyme contains two heme molecules. Presence of protoheme IX, absence of the other heme types. The ratio of protoheme IX to the SQR protomer is 1.5, there are two protoheme IX-binding sites in SQR | Corynebacterium glutamicum | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0009 | - |
2-n-heptyl-4-hydroxyquinoline | purified enzyme | Corynebacterium glutamicum | |
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