Application | Comment | Organism |
---|---|---|
analysis | useful for the determination of free fatty acids | Paenarthrobacter ureafaciens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli JM109 cells | Paenarthrobacter ureafaciens |
into vector pUTE300, expression in Escherichia coli JM109 | Paenarthrobacter ureafaciens |
into vector pUTE300, expression in Escherichia coli JM109 | Arthrobacter sp. |
Protein Variants | Comment | Organism |
---|---|---|
C159T | exhibits 60% activity of the wild-type enzyme, looses more than half of the activity after incubation with N-ethylmaleimide | Paenarthrobacter ureafaciens |
C159T | 60% activity compared to the wild type enzyme and shows increased sensitivity to N-ethylmaleimide | Paenarthrobacter ureafaciens |
C159T/C420S | activity less than one tenth of that of the wild type | Paenarthrobacter ureafaciens |
C159T/C420S/C424V | shows no activity at all | Paenarthrobacter ureafaciens |
C159T/C424V | activity less than one tenth of that of the wild type | Paenarthrobacter ureafaciens |
C420S | exhibits 41% activity of the wild-type enzyme, retains about 90% of the activity after incubation with N-ethylmaleimide | Paenarthrobacter ureafaciens |
C420S | 41% activity compared to the wild type enzyme and shows increased sensitivity to N-ethylmaleimide | Paenarthrobacter ureafaciens |
C420S/424V | activity less than one tenth of that of the wild type | Paenarthrobacter ureafaciens |
C424V | looses more than half of the activity after incubation with N-Ethylmaleimide | Paenarthrobacter ureafaciens |
C424V | 98% activity compared to the wild type enzyme and shows increased sensitivity to N-ethylmaleimide | Paenarthrobacter ureafaciens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
N-ethylmaleimide | shows less than 10% activity in the presence of 10 mM at 37°C for 4 h | Arthrobacter sp. | |
N-ethylmaleimide | shows less than 10% activity in the presence of 10 mM at 37 °C for 4 h | Candida sp. (in: Saccharomycetales) | |
N-ethylmaleimide | ACO retains more than 60% of the initial activity in the presence of 10 mM N-ethylmaleimide, at 37°C for 4 h; purified enzyme retains more than 60% activity in the presence of 10 mM at 37°C for 4 h, while other commercially available ACOs show only less than 10% activities after the same treatment | Paenarthrobacter ureafaciens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00000002 | - |
palmitoyl-CoA | - |
Paenarthrobacter ureafaciens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
75000 | - |
2 * 75000, SDS-PAGE | Paenarthrobacter ureafaciens |
76400 | - |
predicted | Arthrobacter sp. |
76500 | - |
predicted from amino acid sequence | Paenarthrobacter ureafaciens |
95000 | - |
2 * 95000, gel filtration | Paenarthrobacter ureafaciens |
160000 | - |
native-PAGE | Paenarthrobacter ureafaciens |
180000 | - |
SDS-PAGE, recombinant enzyme | Paenarthrobacter ureafaciens |
190000 | - |
gel filtration | Paenarthrobacter ureafaciens |
190000 | - |
gel filtration, recombinant enzyme | Paenarthrobacter ureafaciens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arthrobacter sp. | Q33DR1 | strain 2-4-1 | - |
Arthrobacter sp. 02.04.2001 | Q33DR1 | strain 2-4-1 | - |
Candida sp. (in: Saccharomycetales) | - |
- |
- |
Paenarthrobacter ureafaciens | Q33DR0 | - |
- |
Paenarthrobacter ureafaciens NBRC 12140 | Q33DR0 | - |
- |
Purification (Comment) | Organism |
---|---|
by gel filtration | Paenarthrobacter ureafaciens |
Q-Sepharose column chromatography and CM-Sepharose column chromatography | Paenarthrobacter ureafaciens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.7 | - |
crude enzyme | Paenarthrobacter ureafaciens |
1.7 | - |
crude extract, at 37°C | Paenarthrobacter ureafaciens |
60.9 | - |
purified enzyme | Paenarthrobacter ureafaciens |
60.9 | - |
purified recombinant enzyme, at 37°C | Paenarthrobacter ureafaciens |
Storage Stability | Organism |
---|---|
-20°C, potassium phosphate buffer, pH 7.0 | Paenarthrobacter ureafaciens |
37°C, 0.2 M potassium phosphate (pH 7.5) containing 0.1 M FAD, 4 h, less than 10% loss of activity | Paenarthrobacter ureafaciens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | high activity toward acyl-CoAs with a chain length of C12-C18, inactive with short chain acyl-CoAs with a chain length of C4 and C6 | Paenarthrobacter ureafaciens | ? | - |
? | |
additional information | exhibits high activity towards acyl-CoAs with chain lenghths of C12-C18 | Paenarthrobacter ureafaciens | ? | - |
? | |
additional information | high activity toward acyl-CoAs with a chain length of C12-C18, inactive with short chain acyl-CoAs with a chain length of C4 and C6 | Paenarthrobacter ureafaciens NBRC 12140 | ? | - |
? | |
additional information | exhibits high activity towards acyl-CoAs with chain lenghths of C12-C18 | Paenarthrobacter ureafaciens NBRC 12140 | ? | - |
? | |
myristoyl-CoA + O2 | highest activity | Paenarthrobacter ureafaciens | trans-2-tetradecenoyl-CoA + H2O2 | - |
ir | |
myristoyl-CoA + O2 | maximum activity | Paenarthrobacter ureafaciens | trans-2-tetradecenoyl-CoA + H2O2 | - |
? | |
myristoyl-CoA + O2 | highest activity | Paenarthrobacter ureafaciens NBRC 12140 | trans-2-tetradecenoyl-CoA + H2O2 | - |
ir | |
palmitoyl-CoA + O2 | - |
Paenarthrobacter ureafaciens | 2-trans-hexadecenoyl-CoA + H2O2 | - |
? | |
palmitoyl-CoA + O2 | - |
Paenarthrobacter ureafaciens | 2-trans-hexadecenoyl-CoA + H2O2 | - |
ir | |
palmitoyl-CoA + O2 | - |
Paenarthrobacter ureafaciens NBRC 12140 | 2-trans-hexadecenoyl-CoA + H2O2 | - |
? | |
palmitoyl-CoA + O2 | - |
Paenarthrobacter ureafaciens NBRC 12140 | 2-trans-hexadecenoyl-CoA + H2O2 | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 76500, SDS-PAGE | Paenarthrobacter ureafaciens |
homodimer | 2 * 75000, SDS-PAGE | Paenarthrobacter ureafaciens |
homodimer | 2 * 95000, gel filtration | Paenarthrobacter ureafaciens |
Synonyms | Comment | Organism |
---|---|---|
ACO | - |
Candida sp. (in: Saccharomycetales) |
ACO | - |
Paenarthrobacter ureafaciens |
ACO | - |
Arthrobacter sp. |
ACO-A1 | recombinant enzyme | Paenarthrobacter ureafaciens |
acyl-coenzyme A oxidase | - |
Candida sp. (in: Saccharomycetales) |
acyl-coenzyme A oxidase | - |
Paenarthrobacter ureafaciens |
acyl-coenzyme A oxidase | - |
Arthrobacter sp. |
acyl-coenzyme A: oxygen oxidoreductase | - |
Paenarthrobacter ureafaciens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Paenarthrobacter ureafaciens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
78 | - |
palmitoyl-CoA | - |
Paenarthrobacter ureafaciens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Paenarthrobacter ureafaciens |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
between 6.57.5 at 37°C | Paenarthrobacter ureafaciens |
6.5 | 7.5 | at 37°C | Paenarthrobacter ureafaciens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Candida sp. (in: Saccharomycetales) | |
FAD | - |
Arthrobacter sp. | |
FAD | one molecule of non-covalently bound FAD per subunit as a prosthetic group | Paenarthrobacter ureafaciens | |
FAD | flavoenzyme with 1 mol FAD per subunit | Paenarthrobacter ureafaciens |