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Literature summary for 1.3.3.6 extracted from

  • Bakke, M.; Setoyama, C.; Miura, R.; Kajiyama, N.
    N-Ethylmaleimide-resistant acyl-coenzyme A oxidase from Arthrobacter ureafaciens NBRC 12140: Molecular cloning, gene expression and characterization of the recombinant enzyme (2007), Biochim. Biophys. Acta, 1774, 65-71.
    View publication on PubMed

Application

Application Comment Organism
analysis useful for the determination of free fatty acids Paenarthrobacter ureafaciens

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli JM109 cells Paenarthrobacter ureafaciens
into vector pUTE300, expression in Escherichia coli JM109 Paenarthrobacter ureafaciens
into vector pUTE300, expression in Escherichia coli JM109 Arthrobacter sp.

Protein Variants

Protein Variants Comment Organism
C159T exhibits 60% activity of the wild-type enzyme, looses more than half of the activity after incubation with N-ethylmaleimide Paenarthrobacter ureafaciens
C159T 60% activity compared to the wild type enzyme and shows increased sensitivity to N-ethylmaleimide Paenarthrobacter ureafaciens
C159T/C420S activity less than one tenth of that of the wild type Paenarthrobacter ureafaciens
C159T/C420S/C424V shows no activity at all Paenarthrobacter ureafaciens
C159T/C424V activity less than one tenth of that of the wild type Paenarthrobacter ureafaciens
C420S exhibits 41% activity of the wild-type enzyme, retains about 90% of the activity after incubation with N-ethylmaleimide Paenarthrobacter ureafaciens
C420S 41% activity compared to the wild type enzyme and shows increased sensitivity to N-ethylmaleimide Paenarthrobacter ureafaciens
C420S/424V activity less than one tenth of that of the wild type Paenarthrobacter ureafaciens
C424V looses more than half of the activity after incubation with N-Ethylmaleimide Paenarthrobacter ureafaciens
C424V 98% activity compared to the wild type enzyme and shows increased sensitivity to N-ethylmaleimide Paenarthrobacter ureafaciens

Inhibitors

Inhibitors Comment Organism Structure
N-ethylmaleimide shows less than 10% activity in the presence of 10 mM at 37°C for 4 h Arthrobacter sp.
N-ethylmaleimide shows less than 10% activity in the presence of 10 mM at 37 °C for 4 h Candida sp. (in: Saccharomycetales)
N-ethylmaleimide ACO retains more than 60% of the initial activity in the presence of 10 mM N-ethylmaleimide, at 37°C for 4 h; purified enzyme retains more than 60% activity in the presence of 10 mM at 37°C for 4 h, while other commercially available ACOs show only less than 10% activities after the same treatment Paenarthrobacter ureafaciens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00000002
-
palmitoyl-CoA
-
Paenarthrobacter ureafaciens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
2 * 75000, SDS-PAGE Paenarthrobacter ureafaciens
76400
-
predicted Arthrobacter sp.
76500
-
predicted from amino acid sequence Paenarthrobacter ureafaciens
95000
-
2 * 95000, gel filtration Paenarthrobacter ureafaciens
160000
-
native-PAGE Paenarthrobacter ureafaciens
180000
-
SDS-PAGE, recombinant enzyme Paenarthrobacter ureafaciens
190000
-
gel filtration Paenarthrobacter ureafaciens
190000
-
gel filtration, recombinant enzyme Paenarthrobacter ureafaciens

Organism

Organism UniProt Comment Textmining
Arthrobacter sp. Q33DR1 strain 2-4-1
-
Arthrobacter sp. 02.04.2001 Q33DR1 strain 2-4-1
-
Candida sp. (in: Saccharomycetales)
-
-
-
Paenarthrobacter ureafaciens Q33DR0
-
-
Paenarthrobacter ureafaciens NBRC 12140 Q33DR0
-
-

Purification (Commentary)

Purification (Comment) Organism
by gel filtration Paenarthrobacter ureafaciens
Q-Sepharose column chromatography and CM-Sepharose column chromatography Paenarthrobacter ureafaciens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7
-
crude enzyme Paenarthrobacter ureafaciens
1.7
-
crude extract, at 37°C Paenarthrobacter ureafaciens
60.9
-
purified enzyme Paenarthrobacter ureafaciens
60.9
-
purified recombinant enzyme, at 37°C Paenarthrobacter ureafaciens

Storage Stability

Storage Stability Organism
-20°C, potassium phosphate buffer, pH 7.0 Paenarthrobacter ureafaciens
37°C, 0.2 M potassium phosphate (pH 7.5) containing 0.1 M FAD, 4 h, less than 10% loss of activity Paenarthrobacter ureafaciens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information high activity toward acyl-CoAs with a chain length of C12-C18, inactive with short chain acyl-CoAs with a chain length of C4 and C6 Paenarthrobacter ureafaciens ?
-
?
additional information exhibits high activity towards acyl-CoAs with chain lenghths of C12-C18 Paenarthrobacter ureafaciens ?
-
?
additional information high activity toward acyl-CoAs with a chain length of C12-C18, inactive with short chain acyl-CoAs with a chain length of C4 and C6 Paenarthrobacter ureafaciens NBRC 12140 ?
-
?
additional information exhibits high activity towards acyl-CoAs with chain lenghths of C12-C18 Paenarthrobacter ureafaciens NBRC 12140 ?
-
?
myristoyl-CoA + O2 highest activity Paenarthrobacter ureafaciens trans-2-tetradecenoyl-CoA + H2O2
-
ir
myristoyl-CoA + O2 maximum activity Paenarthrobacter ureafaciens trans-2-tetradecenoyl-CoA + H2O2
-
?
myristoyl-CoA + O2 highest activity Paenarthrobacter ureafaciens NBRC 12140 trans-2-tetradecenoyl-CoA + H2O2
-
ir
palmitoyl-CoA + O2
-
Paenarthrobacter ureafaciens 2-trans-hexadecenoyl-CoA + H2O2
-
?
palmitoyl-CoA + O2
-
Paenarthrobacter ureafaciens 2-trans-hexadecenoyl-CoA + H2O2
-
ir
palmitoyl-CoA + O2
-
Paenarthrobacter ureafaciens NBRC 12140 2-trans-hexadecenoyl-CoA + H2O2
-
?
palmitoyl-CoA + O2
-
Paenarthrobacter ureafaciens NBRC 12140 2-trans-hexadecenoyl-CoA + H2O2
-
ir

Subunits

Subunits Comment Organism
? x * 76500, SDS-PAGE Paenarthrobacter ureafaciens
homodimer 2 * 75000, SDS-PAGE Paenarthrobacter ureafaciens
homodimer 2 * 95000, gel filtration Paenarthrobacter ureafaciens

Synonyms

Synonyms Comment Organism
ACO
-
Candida sp. (in: Saccharomycetales)
ACO
-
Paenarthrobacter ureafaciens
ACO
-
Arthrobacter sp.
ACO-A1 recombinant enzyme Paenarthrobacter ureafaciens
acyl-coenzyme A oxidase
-
Candida sp. (in: Saccharomycetales)
acyl-coenzyme A oxidase
-
Paenarthrobacter ureafaciens
acyl-coenzyme A oxidase
-
Arthrobacter sp.
acyl-coenzyme A: oxygen oxidoreductase
-
Paenarthrobacter ureafaciens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
-
Paenarthrobacter ureafaciens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
78
-
palmitoyl-CoA
-
Paenarthrobacter ureafaciens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Paenarthrobacter ureafaciens

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
between 6.5–7.5 at 37°C Paenarthrobacter ureafaciens
6.5 7.5 at 37°C Paenarthrobacter ureafaciens

Cofactor

Cofactor Comment Organism Structure
FAD
-
Candida sp. (in: Saccharomycetales)
FAD
-
Arthrobacter sp.
FAD one molecule of non-covalently bound FAD per subunit as a prosthetic group Paenarthrobacter ureafaciens
FAD flavoenzyme with 1 mol FAD per subunit Paenarthrobacter ureafaciens