Application | Comment | Organism |
---|---|---|
additional information | isomerase activity of rat peroxisomal acyl-CoA oxidase I, is probably due to a spontaneous process driven by thermodynamic equilibrium with formation of a conjugated structure after deprotonation of substrate alpha-proton | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
His-tagged | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
E421A | does not show any isomerase activity | Rattus norvegicus |
E421D | isomerase activity is decreased for all tested cis- and trans-substrates compared with that of wild-type enzyme | Rattus norvegicus |
E421Q | does not show any isomerase activity | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.044 | - |
cis-3-decenoyl-CoA | mutant E421D | Rattus norvegicus | |
0.054 | - |
cis-3-decenoyl-CoA | wild-type | Rattus norvegicus | |
0.054 | - |
cis-3-octenoyl-CoA | mutant E421D | Rattus norvegicus | |
0.056 | - |
trans-3-decenoyl-CoA | wild-type | Rattus norvegicus | |
0.057 | - |
cis-3-octenoyl-CoA | wild-type | Rattus norvegicus | |
0.059 | - |
trans-3-octenoyl-CoA | wild-type | Rattus norvegicus | |
0.063 | - |
trans-3-hexenoyl-CoA | wild-type | Rattus norvegicus | |
0.063 | - |
cis-3-hexenoyl-CoA | wild-type | Rattus norvegicus | |
0.064 | - |
trans-3-decenoyl-CoA | mutant E421D | Rattus norvegicus | |
0.066 | - |
trans-3-octenoyl-CoA | mutant E421D | Rattus norvegicus | |
0.076 | - |
cis-3-hexenoyl-CoA | mutant E421D | Rattus norvegicus | |
0.083 | - |
trans-3-hexenoyl-CoA | mutant E421D | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Rattus norvegicus | 5777 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
cis-3-decenoyl-CoA + O2 | - |
Rattus norvegicus | ? | - |
? | |
cis-3-hexenoyl-CoA + O2 | best substrate for the isomerase activity of the enzyme | Rattus norvegicus | ? | - |
? | |
cis-3-octenoyl-CoA + O2 | - |
Rattus norvegicus | ? | - |
? | |
trans-3-decenoyl-CoA + O2 | - |
Rattus norvegicus | ? | - |
? | |
trans-3-hexenoyl-CoA + O2 | - |
Rattus norvegicus | ? | - |
? | |
trans-3-octenoyl-CoA + O2 | - |
Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACO I | - |
Rattus norvegicus |
acyl-CoA oxidase I | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0053 | - |
trans-3-decenoyl-CoA | mutant E421D | Rattus norvegicus | |
0.012 | - |
trans-3-octenoyl-CoA | mutant E421D | Rattus norvegicus | |
0.01567 | - |
trans-3-decenoyl-CoA | wild-type | Rattus norvegicus | |
0.0283 | - |
trans-3-hexenoyl-CoA | mutant E421D | Rattus norvegicus | |
0.04 | - |
cis-3-decenoyl-CoA | mutant E421D | Rattus norvegicus | |
0.045 | - |
trans-3-octenoyl-CoA | wild-type | Rattus norvegicus | |
0.05 | - |
trans-3-hexenoyl-CoA | wild-type | Rattus norvegicus | |
0.053 | - |
cis-3-octenoyl-CoA | mutant E421D | Rattus norvegicus | |
0.06167 | - |
cis-3-decenoyl-CoA | wild-type | Rattus norvegicus | |
0.075 | - |
cis-3-octenoyl-CoA | wild-type | Rattus norvegicus | |
0.165 | - |
cis-3-hexenoyl-CoA | mutant E421D | Rattus norvegicus | |
0.35 | - |
cis-3-hexenoyl-CoA | wild-type | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | required for the isomerase activity of the enzyme | Rattus norvegicus |