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Literature summary for 1.3.3.6 extracted from

  • Zeng, J.; Li, D.
    Expression and purification of his-tagged rat peroxisomal acyl-CoA oxidase I wild-type and E421 mutant proteins (2004), Protein Expr. Purif., 38, 153-160.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
cloned into a bacterial expression vector pLM1 with six continous His codons attached to the 5' end of the gene, overexpression of wild-type ands mutant enzymes E421D, E421A, E421Q and E421G in Escherichia coli Rattus norvegicus

Protein Variants

Protein Variants Comment Organism
E421A inactive mutant enzyme Rattus norvegicus
E421D Km-value for octanoyl-CoA is 1.23fold higher than the wild-type value. The turnover number for octanoyl-CoA is 1.6fold lower than activity of the wild-type enzyme Rattus norvegicus
E421G inactive mutant enzyme Rattus norvegicus
E421Q inactive mutant enzyme Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0443
-
octanoyl-CoA pH 7.4, 30°C, wild-type enzyme Rattus norvegicus
0.0546
-
octanoyl-CoA pH 7.4, 30°C, mutant enzyme E421D Rattus norvegicus

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisome
-
Rattus norvegicus 5777
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus key enzyme for the beta-oxidation of fatty acids ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Rattus norvegicus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5
-
-
Rattus norvegicus

Storage Stability

Storage Stability Organism
-80°C, wild-type enzyme and mutant enzymes E421D, E421A, E421Q and E421G are stable for 3 months Rattus norvegicus
4°C, His-tagged enzyme is stable for 1 week Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information key enzyme for the beta-oxidation of fatty acids Rattus norvegicus ?
-
?
octanoyl-CoA + O2
-
Rattus norvegicus trans-2-octenoyl-CoA + H2O2
-
?

Synonyms

Synonyms Comment Organism
ACO I
-
Rattus norvegicus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.18
-
octanoyl-CoA pH 7.4, 30°C, mutant enzyme E421D Rattus norvegicus
3.47
-
octanoyl-CoA pH 7.4, 30°C, wild-type enzyme Rattus norvegicus