Cloned (Comment) | Organism |
---|---|
cloned into a bacterial expression vector pLM1 with six continous His codons attached to the 5' end of the gene, overexpression of wild-type ands mutant enzymes E421D, E421A, E421Q and E421G in Escherichia coli | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
E421A | inactive mutant enzyme | Rattus norvegicus |
E421D | Km-value for octanoyl-CoA is 1.23fold higher than the wild-type value. The turnover number for octanoyl-CoA is 1.6fold lower than activity of the wild-type enzyme | Rattus norvegicus |
E421G | inactive mutant enzyme | Rattus norvegicus |
E421Q | inactive mutant enzyme | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0443 | - |
octanoyl-CoA | pH 7.4, 30°C, wild-type enzyme | Rattus norvegicus | |
0.0546 | - |
octanoyl-CoA | pH 7.4, 30°C, mutant enzyme E421D | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Rattus norvegicus | 5777 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Rattus norvegicus | key enzyme for the beta-oxidation of fatty acids | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rattus norvegicus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.5 | - |
- |
Rattus norvegicus |
Storage Stability | Organism |
---|---|
-80°C, wild-type enzyme and mutant enzymes E421D, E421A, E421Q and E421G are stable for 3 months | Rattus norvegicus |
4°C, His-tagged enzyme is stable for 1 week | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | key enzyme for the beta-oxidation of fatty acids | Rattus norvegicus | ? | - |
? | |
octanoyl-CoA + O2 | - |
Rattus norvegicus | trans-2-octenoyl-CoA + H2O2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ACO I | - |
Rattus norvegicus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.18 | - |
octanoyl-CoA | pH 7.4, 30°C, mutant enzyme E421D | Rattus norvegicus | |
3.47 | - |
octanoyl-CoA | pH 7.4, 30°C, wild-type enzyme | Rattus norvegicus |