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Literature summary for 1.3.3.6 extracted from

  • Shimizu, S.; Yasui, K.; Tani, Y.; Yamada, H.
    Acyl-CoA oxidase from Candida tropicalis (1979), Biochem. Biophys. Res. Commun., 91, 108-113.
    View publication on PubMed
Search for more literature for 1.3.3.6

Crystallization (Commentary)

Crystallization (Comment) Organism
at 5°C from a 30% saturated ammonium sulfate solution, yellow rod-shaped crystals Candida tropicalis

Inhibitors

Inhibitors Comment Organism Structure
AgNO3
-
 Candida tropicalis
HgCl2
-
 Candida tropicalis
Mercuric acetate
-
 Candida tropicalis
additional information glutathione protects against inhibition with sulfhydryl reagents Candida tropicalis
p-chloromercuribenzoate
-
 Candida tropicalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.024
-
 decanoyl-CoA
-
 Candida tropicalis
0.024
-
 lauroyl-CoA
-
 Candida tropicalis
0.029
-
 myristoyl-CoA
-
 Candida tropicalis
0.0335
-
 arachidoyl-CoA
-
 Candida tropicalis
0.034
-
 stearoyl-CoA
-
 Candida tropicalis
0.042
-
 octanoyl-CoA
-
 Candida tropicalis
0.046
-
 oleoyl-CoA
-
 Candida tropicalis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
75000
-
 8 * 75000, SDS-PAGE Candida tropicalis
600000
-
 sedimentation equilibrium Candida tropicalis

Organism

Organism UniProt Comment Textmining
Candida tropicalis
-
 pK 233, inducible by growth on alkanes
-

Purification (Commentary)

Purification (Comment) Organism
-
 Candida tropicalis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
19.13
-
-
 Candida tropicalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
acyl-CoA + O2 specificity: C4-C20 acyl-CoA Candida tropicalis trans-2,3-dehydroacyl-CoA + H2O2
-
 ?
arachidoyl-CoA + O2
-
 Candida tropicalis 2-trans-eicosenoyl-CoA + H2O2
-
 ?
decanoyl-CoA + O2
-
 Candida tropicalis trans-2-decenoyl-CoA + H2O2
-
 ?
lauroyl-CoA + O2
-
 Candida tropicalis trans-2-dodecenoyl-CoA + H2O2
-
 ?
myristoyl-CoA + O2
-
 Candida tropicalis trans-2-tetradecenoyl-CoA + H2O2
-
 ?
octanoyl-CoA + O2
-
 Candida tropicalis trans-2-octenoyl-CoA + H2O2
-
 ?
oleoyl-CoA + O2
-
 Candida tropicalis 2-trans-9-trans-octadecendienoyl-CoA + H2O2
-
 ?
palmitoyl-CoA + O2
-
 Candida tropicalis 2-trans-hexadecenoyl-CoA + H2O2
-
 ?
stearoyl-CoA + O2
-
 Candida tropicalis trans-2-octadecenoyl-CoA + H2O2
-
 ?

Subunits

Subunits Comment Organism
octamer 8 * 75000, SDS-PAGE Candida tropicalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Candida tropicalis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 10 min Candida tropicalis
65
-
 10 min, complete inactivation Candida tropicalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Candida tropicalis

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 9 35°C, 60 min Candida tropicalis

Cofactor

Cofactor Comment Organism Structure
FAD flavoprotein Candida tropicalis
FAD 8 mol FAD per mol of enzyme, 1 mol FAD per mol of subunit Candida tropicalis