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Literature summary for 1.3.3.5 extracted from
- Bilirubin oxidase activity of Bacillus subtilis CotA (2006), Appl. Environ. Microbiol., 72, 972-975.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | great potential for use as a diagnostic reagent, can be used for accurate determination of serum bilirubin levels in patients with hepatobiliary disease | Bacillus subtilis |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Escherichia coli JM109 transformed with pUC119 carrying the CotA gene, produces large amounts of the soluble protein under low-temperature conditions | Bacillus subtilis |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.008 | - |
bilirubin | - |
Bacillus subtilis |  |
| 0.015 | - |
ditaurobilirubin | - |
Bacillus subtilis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Bacillus subtilis | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus subtilis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by gel filtration | Bacillus subtilis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| bilirubin + O2 | CotA with markedly higher affinity for bilirubin than conventional bilirubin oxidase | Bacillus subtilis | biliverdin + H2O | - |
? | |
| ditaurobilirubin + O2 | - |
Bacillus subtilis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CotA | - |
Bacillus subtilis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 21 | 37 | at 24°C approximately 50% of the CotA protein is detected in the soluble fraction | Bacillus subtilis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 65 | - |
stable for 60 min | Bacillus subtilis |
| 84 | - |
stable for 30 min | Bacillus subtilis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4 | - |
optimal for ditaurobilirubin oxidation | Bacillus subtilis |
| 7 | - |
optimal for bilirubin oxidation | Bacillus subtilis |
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Release 2023.1 (January 2023)
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