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Literature summary for 1.3.3.5 extracted from

  • Skrika-Alexopoulos, E.; Freedman, R.B.
    Factors affecting enzyme characteristics of bilirubin oxidase suspensions in organic solvents (1993), Biotechnol. Bioeng., 41, 887-893.
    View publication on PubMed
Search for more literature for 1.3.3.5

Inhibitors

Inhibitors Comment Organism Structure
bilirubin 0.09 mM, complete inhibition of enzyme activity in 50 : 50 chloroform-n-heptane two-phase system Albifimbria verrucaria

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0602
-
 bilirubin preparations of enzyme lyophilized from N-(2-hydroxyethyl)-piperazine-N'-(3-propane-sulfonic acid) buffer suspended in 50 : 50 v/v chloroform-n-heptane mixture Albifimbria verrucaria
0.19
-
 bilirubin assay in purely aqueous system Albifimbria verrucaria

Organic Solvent Stability

Organic Solvent Comment Organism
chloroform chloroform-n-heptane mixture, preparations of enzyme lyophilized from N-(2-hydroxyethyl)-piperazine-N'-(3-propane-sulfonic acid) buffer suspended in 50:50 v/v chloroform-n-heptane mixture are activ if more than 0.081% v/v water are present in the two-phase system Albifimbria verrucaria
n-heptane chloroform-n-heptane mixture, preparations of enzyme lyophilized from N-(2-hydroxyethyl)-piperazine-N'-(3-propane-sulfonic acid) buffer suspended in 50:50 v/v chloroform-n-heptane mixture are active if more than 0.081% v/v water are present in the two-phase system Albifimbria verrucaria

Organism

Organism UniProt Comment Textmining
Albifimbria verrucaria
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
bilirubin + O2
-
 Albifimbria verrucaria biliverdin + H2O
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00133
-
 bilirubin preparations of enzyme lyophilized from N-(2-hydroxyethyl)-piperazine-N'-(3-propane-sulfonic acid) buffer suspended in 50 : 50 v/v chloroform-n-heptane mixture Albifimbria verrucaria