Cloned (Comment) | Organism |
---|---|
the enzyme gene is fused in its 3'-end to a polynucleotide encoding six histidine residues and expressed in both Escherichia coli BL21(DE3) cells and Bacillus subtilis 168A cells | Bacillus subtilis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
53000 | - |
x * 53000, SDS-PAGE | Bacillus subtilis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen IX + O2 | Bacillus subtilis | 20fold lower activity with protoporphyrinogen IX compared to coproporphyrinogen III | protoporphyrin IX + H2O | - |
? | |
protoporphyrinogen IX + O2 | Bacillus subtilis 168 | 20fold lower activity with protoporphyrinogen IX compared to coproporphyrinogen III | protoporphyrin IX + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P32397 | - |
- |
Bacillus subtilis 168 | P32397 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+ affinity column chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen IX + O2 | 20fold lower activity with protoporphyrinogen IX compared to coproporphyrinogen III | Bacillus subtilis | protoporphyrin IX + H2O | - |
? | |
protoporphyrinogen IX + O2 | 20fold lower activity with protoporphyrinogen IX compared to coproporphyrinogen III | Bacillus subtilis 168 | protoporphyrin IX + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 53000, SDS-PAGE | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
hemY | - |
Bacillus subtilis |
protoporphyrinogen IX oxidase | - |
Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Bacillus subtilis |