Crystallization (Comment) | Organism |
---|---|
purified native and SeMet-labeled enzyme in complex with inhibitor acifluorfen, sitting-drop, vapor-diffusion method, 10 mg/ml wild-type bsPPO protein in 50 mM TrisHCl, pH 7.5, 200 mM sodium chloride, 5 mM DTT, 1 mM EDTA, and acifluorfen in a 1:5 ratio, equilibrating against a reservoir solution of 2.4 M ammonium phosphate dibasic, 0.1 M Tris-HCl, pH 7.5, 1 week, 20°C, X-ray diffraction structure determination and analysis at 2.8-3.5 A resolution | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
F227R | the mutant shows reduced activity compared to the wild-type enzyme | Bacillus subtilis |
I176A | the mutant shows reduced activity compared to the wild-type enzyme | Bacillus subtilis |
K71A | the mutant shows reduced activity compared to the wild-type enzyme | Bacillus subtilis |
P64A | the mutant shows reduced activity compared to the wild-type enzyme | Bacillus subtilis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acifluorfen | a herbicide, binding structure, overview | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.001 | - |
protoporphyrinogen IX | mutant F227R, pH 7.5, temperature not specified in the publication | Bacillus subtilis | |
0.001 | - |
protoporphyrinogen IX | mutant I176A, pH 7.5, temperature not specified in the publication | Bacillus subtilis | |
0.001 | - |
protoporphyrinogen IX | mutant K71A, pH 7.5, temperature not specified in the publication | Bacillus subtilis | |
0.001 | - |
protoporphyrinogen IX | mutant P64A, pH 7.5, temperature not specified in the publication | Bacillus subtilis | |
0.001 | - |
protoporphyrinogen IX | wild-type enzyme, pH 7.5, temperature not specified in the publication | Bacillus subtilis |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | Bacillus subtilis PPO is unique among PPO family members in that it is a soluble monomer | Bacillus subtilis | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen IX + 3 O2 | Bacillus subtilis | - |
protoporphyrin IX + 3 H2O2 | - |
? | |
protoporphyrinogen IX + 3 O2 | Bacillus subtilis 168 | - |
protoporphyrin IX + 3 H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | P32397 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | bsPPO has a broader substrate specificity compared to other PPOs, substrate binding structure comparison of wild-type and mutant enzymes, overview | Bacillus subtilis | ? | - |
? | |
additional information | bsPPO has a broader substrate specificity compared to other PPOs, substrate binding structure comparison of wild-type and mutant enzymes, overview | Bacillus subtilis 168 | ? | - |
? | |
protoporphyrinogen IX + 3 O2 | - |
Bacillus subtilis | protoporphyrin IX + 3 H2O2 | - |
? | |
protoporphyrinogen IX + 3 O2 | - |
Bacillus subtilis 168 | protoporphyrin IX + 3 H2O2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | Bacillus subtilis PPO is unique among PPO family members in that it is a soluble monomer | Bacillus subtilis |
More | prototypical PPO enzyme structure consists of three domains: a FAD-binding domain, a substrate binding domain and a membrane-binding domain, structure analysis and comparison, overview | Bacillus subtilis |
Synonyms | Comment | Organism |
---|---|---|
PPO | - |
Bacillus subtilis |
protoporphyrinogen IX oxidase | - |
Bacillus subtilis |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0004 | - |
protoporphyrinogen IX | mutant K71A, pH 7.5, temperature not specified in the publication | Bacillus subtilis | |
0.001 | - |
protoporphyrinogen IX | mutant I176A, pH 7.5, temperature not specified in the publication | Bacillus subtilis | |
0.0027 | - |
protoporphyrinogen IX | mutant P64A, pH 7.5, temperature not specified in the publication | Bacillus subtilis | |
0.0131 | - |
protoporphyrinogen IX | wild-type enzyme, pH 7.5, temperature not specified in the publication | Bacillus subtilis | |
0.0181 | - |
protoporphyrinogen IX | mutant F227R, pH 7.5, temperature not specified in the publication | Bacillus subtilis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Bacillus subtilis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | a FAD molecule is non-covalently bound to domain I, binding structure, overview | Bacillus subtilis |
General Information | Comment | Organism |
---|---|---|
metabolism | PPO plays an important part in the heme/chlorophyll biosynthetic pathway | Bacillus subtilis |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.016 | - |
protoporphyrinogen IX | mutant K71A, pH 7.5, temperature not specified in the publication | Bacillus subtilis | |
0.027 | - |
protoporphyrinogen IX | mutant I176A, pH 7.5, temperature not specified in the publication | Bacillus subtilis | |
0.057 | - |
protoporphyrinogen IX | mutant P64A, pH 7.5, temperature not specified in the publication | Bacillus subtilis | |
0.464 | - |
protoporphyrinogen IX | mutant F227R, pH 7.5, temperature not specified in the publication | Bacillus subtilis | |
0.784 | - |
protoporphyrinogen IX | wild-type enzyme, pH 7.5, temperature not specified in the publication | Bacillus subtilis |