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Literature summary for 1.3.3.4 extracted from

  • Tan, Y.; Sun, L.; Xi, Z.; Yang, G.F.; Jiang, D.Q.; Yan, X.P.; Yang, X.; Li, H.Y.
    A capillary electrophoresis assay for recombinant Bacillus subtilis protoporphyrinogen oxidase (2008), Anal. Biochem., 383, 200-204.
    View publication on PubMed
Search for more literature for 1.3.3.4

Application

Application Comment Organism
drug development protoporphyrinogen oxidase is one of the most important action targets of commercial herbicides Bacillus subtilis

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged enzyme in Escherichia coli Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
acifluorfen competitive inhibition with respect to protoporphyrinogen IX Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information steady-state kinetics, overview Bacillus subtilis
0.007
-
 protoporphyrinogen IX pH 7.5, 25°C Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoporphyrinogen IX + 3 O2 Bacillus subtilis
-
 protoporphyrin + 3 H2O2
-
 ?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Bacillus subtilis

Source Tissue

Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
protoporphyrinogen IX + 3 O2
-
 Bacillus subtilis protoporphyrin + 3 H2O2
-
 ?
protoporphyrinogen IX + 3 O2 PPO catalyzes the oxygen-dependent six-electron oxidation of protoporphyrinogen IX, i.e. protogen IX, to the fully conjugated macrocycle of protoporphyrin IX, i.e. proto IX Bacillus subtilis protoporphyrin + 3 H2O2
-
 ?

Synonyms

Synonyms Comment Organism
PPO
-
 Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
FAD protoporphyrinogen oxidase is a FAD-containing enzyme Bacillus subtilis

General Information

General Information Comment Organism
malfunction when PPO is inhibited, protoporphyrin IX accumulates to cause light-dependent membrane damage Bacillus subtilis
metabolism protoporphyrinogen oxidase acts in the tetrapyrrole biosynthetic pathway that leads to the formation of both heme and chlorophylls Bacillus subtilis
physiological function protoporphyrinogen oxidase is one of the most important action targets of commercial herbicides Bacillus subtilis