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Literature summary for 1.3.3.4 extracted from

  • Corrigall, A.V.; Siziba, K.B.; Maneli, M.H.; Shephard, E.G.; Ziman, M.; Dailey, T.A.; Dailey, H.A.; Kirsch, R.E.; Meissner, P.N.
    Purification of and kinetic studies on a cloned protoporphyrinogen oxidase from the aerobic bacterium Bacillus subtilis (1998), Arch. Biochem. Biophys., 358, 251-256.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli, His-tag inserted for purification Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
acifluorfen no inhibition at 0.1 mM Bacillus subtilis
acifluorfen-methyl weak Bacillus subtilis
bilirubin competitive Bacillus subtilis
Biliverdin competitive Bacillus subtilis
hemin competitive Bacillus subtilis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.001
-
protoporphyrinogen-IX
-
Bacillus subtilis
0.0049
-
Mesoporphyrinogen-IX
-
Bacillus subtilis
0.0053
-
Coproporphyrinogen-III
-
Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
54000
-
gel filtration Bacillus subtilis
56000
-
SDS-PAGE Bacillus subtilis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoporphyrinogen-IX + 3 O2 Bacillus subtilis penultimate step of heme and chlorophyll biosynthesis protoporphyrin-IX + 3 H2O2
-
?

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cloned enzyme Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coproporphyrinogen-III + 3 O2
-
Bacillus subtilis coproporphyrin-III + 3 H2O2
-
?
mesoporphyrinogen-IX + 3 O2
-
Bacillus subtilis mesoporphyrin-IX + 3 H2O2
-
?
protoporphyrinogen-IX + 3 O2
-
Bacillus subtilis protoporphyrin-IX + 3 H2O2
-
?
protoporphyrinogen-IX + 3 O2 penultimate step of heme and chlorophyll biosynthesis Bacillus subtilis protoporphyrin-IX + 3 H2O2
-
?

Subunits

Subunits Comment Organism
monomer gel filtration, SDS-PAGE Bacillus subtilis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.000833
-
Coproporphyrinogen-III
-
Bacillus subtilis
0.00317
-
protoporphyrinogen-IX
-
Bacillus subtilis
0.0448
-
Mesoporphyrinogen-IX
-
Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.7
-
-
Bacillus subtilis

Cofactor

Cofactor Comment Organism Structure
FAD noncovalently bound Bacillus subtilis

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3
-
hemin
-
Bacillus subtilis
4
-
Biliverdin
-
Bacillus subtilis
4.4
-
bilirubin
-
Bacillus subtilis