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Literature summary for 1.3.3.4 extracted from
- Human protoporphyrinogen oxidase: Expression, purification, and characterization of the cloned enzyme (1996), Protein Sci., 5, 98-105.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| in Escherichia coli, His-tag inserted for purification | Homo sapiens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| acifluorfen | - |
Homo sapiens |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0017 | - |
protoporphyrinogen-IX | - |
Homo sapiens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51000 | - |
SDS-PAGE | Homo sapiens |
| 100000 | - |
gel filtration | Homo sapiens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrinogen-IX + 3 O2 | Homo sapiens | penultimate step of heme and chlorophyll biosynthesis | protoporphyrin-IX + 3 H2O2 | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| cloned enzyme | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| placenta | - |
Homo sapiens | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, stable for days, slow proteolysis | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| protoporphyrinogen-IX + 3 O2 | - |
Homo sapiens | protoporphyrin-IX + 3 H2O2 | - |
? | |
| protoporphyrinogen-IX + 3 O2 | penultimate step of heme and chlorophyll biosynthesis | Homo sapiens | protoporphyrin-IX + 3 H2O2 | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 51000, SDS-PAGE, gel filtration | Homo sapiens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.175 | - |
protoporphyrinogen-IX | - |
Homo sapiens | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | one noncovalently bound FAD per dimer | Homo sapiens | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
acifluorfen | - |
Homo sapiens | |
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