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Literature summary for 1.3.3.4 extracted from

  • Jacobs, J.M.; Jacobs, N.J.
    Oxidation of protoporphyrinogen to protoporphyrin, a step in chlorophyll and haem biosynthesis. Purification and partial characterization of the enzyme from barley organelles (1987), Biochem. J., 244, 219-224.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
oleic acid
-
Hordeum vulgare

General Stability

General Stability Organism
insensitive to trypsin digestion Hordeum vulgare

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0005
-
protoporphyrinogen-IX
-
Hordeum vulgare

Localization

Localization Comment Organism GeneOntology No. Textmining
etioplast
-
Hordeum vulgare 9513
-
mitochondrion
-
Hordeum vulgare 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
SDS-PAGE Hordeum vulgare
210000
-
gel filtration Hordeum vulgare

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
protoporphyrinogen-IX + 3 O2 Hordeum vulgare penultimate step of heme and chlorophyll biosynthesis protoporphyrin-IX + 3 H2O2
-
?

Organism

Organism UniProt Comment Textmining
Hordeum vulgare
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.012
-
purified enzyme from etioplasts Hordeum vulgare
0.015
-
purified enzyme from mitochondria Hordeum vulgare

Storage Stability

Storage Stability Organism
-20°C, in presence of 20% glycerol, several months, minimal loss of activity Hordeum vulgare

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coproporphyrinogen-I + 3 O2 oxidized at less than 11% of the rate of protoporphyrinogen-IX Hordeum vulgare coproporphyrin-I + 3 H2O2
-
?
mesoporphyrinogen-IX + 3 O2 oxidized at the same rate as protoporphyrinogen-IX Hordeum vulgare mesoporphyrin-IX + 3 H2O2
-
?
protoporphyrinogen-IX + 3 O2
-
Hordeum vulgare protoporphyrin-IX + 3 H2O2
-
?
protoporphyrinogen-IX + 3 O2 penultimate step of heme and chlorophyll biosynthesis Hordeum vulgare protoporphyrin-IX + 3 H2O2
-
?
uroporphyrinogen-I + 3 O2 oxidized at less than 11% of the rate of protoporphyrinogen-IX Hordeum vulgare uroporphyrin-I + 3 H2O2
-
?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
60 min at 55°C causes 80% loss of activity Hordeum vulgare

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Hordeum vulgare

pH Range

pH Minimum pH Maximum Comment Organism
2 8 at pH 2 and pH 10 less than 10% of activity Hordeum vulgare

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
unstable on acidification Hordeum vulgare