Activating Compound | Comment | Organism | Structure |
---|---|---|---|
oleic acid | - |
Hordeum vulgare |
General Stability | Organism |
---|---|
insensitive to trypsin digestion | Hordeum vulgare |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0005 | - |
protoporphyrinogen-IX | - |
Hordeum vulgare |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
etioplast | - |
Hordeum vulgare | 9513 | - |
mitochondrion | - |
Hordeum vulgare | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36000 | - |
SDS-PAGE | Hordeum vulgare |
210000 | - |
gel filtration | Hordeum vulgare |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
protoporphyrinogen-IX + 3 O2 | Hordeum vulgare | penultimate step of heme and chlorophyll biosynthesis | protoporphyrin-IX + 3 H2O2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Hordeum vulgare | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.012 | - |
purified enzyme from etioplasts | Hordeum vulgare |
0.015 | - |
purified enzyme from mitochondria | Hordeum vulgare |
Storage Stability | Organism |
---|---|
-20°C, in presence of 20% glycerol, several months, minimal loss of activity | Hordeum vulgare |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
coproporphyrinogen-I + 3 O2 | oxidized at less than 11% of the rate of protoporphyrinogen-IX | Hordeum vulgare | coproporphyrin-I + 3 H2O2 | - |
? | |
mesoporphyrinogen-IX + 3 O2 | oxidized at the same rate as protoporphyrinogen-IX | Hordeum vulgare | mesoporphyrin-IX + 3 H2O2 | - |
? | |
protoporphyrinogen-IX + 3 O2 | - |
Hordeum vulgare | protoporphyrin-IX + 3 H2O2 | - |
? | |
protoporphyrinogen-IX + 3 O2 | penultimate step of heme and chlorophyll biosynthesis | Hordeum vulgare | protoporphyrin-IX + 3 H2O2 | - |
? | |
uroporphyrinogen-I + 3 O2 | oxidized at less than 11% of the rate of protoporphyrinogen-IX | Hordeum vulgare | uroporphyrin-I + 3 H2O2 | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
55 | - |
60 min at 55°C causes 80% loss of activity | Hordeum vulgare |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Hordeum vulgare |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
2 | 8 | at pH 2 and pH 10 less than 10% of activity | Hordeum vulgare |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
unstable on acidification | Hordeum vulgare |