Literature summary for 1.3.3.3 extracted from

Anand, S.; Rollakanti, K.R.; Brankov, N.; Brash, D.E.; Hasan, T.; Maytin, E.V.
Fluorouracil enhances photodynamic therapy of squamous cell carcinoma via a p53-independent mechanism that increases protoporphyrin IX levels and tumor cell death (2017), Mol. Cancer Ther., 16, 1092-1101 .

Search for more literature for 1.3.3.3

Application

Application	Comment	Organism
medicine	the regulation of the enzyme is important in differentiation therapy for cancer treatment	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
DNA and amino acid sequence determination and analysis, eighteen functional C/EBP binding motifs in the mCPO promoter are found, all are predicted to bind C/EBPs with moderate or higher affinity	Homo sapiens
DNA and amino acid sequence determination and analysis, the murine CPO gene upstream region contains many competent C/EBP binding sites	Mus musculus

Protein Variants

Protein Variants	Comment	Organism
additional information	loss of inducibility due to the promoter mutations occur reproducibly with all C/EBP isoforms, arguing that CPO promoter activity depends upon C/EBP site location, and not simply upon inherent binding affinity, hypothetical modeling, overview	Homo sapiens
additional information	loss of inducibility due to the promoter mutations occur reproducibly with all C/EBP isoforms, arguing that CPO promoter activity depends upon C/EBP site location, and not simply upon inherent binding affinity, hypothetical modeling, overview	Mus musculus

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cytosol	-	Homo sapiens	5829	-
cytosol	-	Mus musculus	5829	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
coproporphyrinogen III + O2 + 2 H+	Homo sapiens	-	protoporphyrinogen-IX + 2 CO2 + 2 H2O	-	?
coproporphyrinogen III + O2 + 2 H+	Mus musculus	-	protoporphyrinogen-IX + 2 CO2 + 2 H2O	-	?

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	P36551	-	-
Mus musculus	P36552	nude mice	-

Source Tissue

Source Tissue	Comment	Organism	Textmining
A-431 cell	-	Mus musculus	-
LNCaP cell	-	Homo sapiens	-
MEL cell	-	Homo sapiens	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
coproporphyrinogen III + O2 + 2 H+	-	Homo sapiens	protoporphyrinogen-IX + 2 CO2 + 2 H2O	-	?
coproporphyrinogen III + O2 + 2 H+	-	Mus musculus	protoporphyrinogen-IX + 2 CO2 + 2 H2O	-	?

Synonyms

Synonyms	Comment	Organism
coproporphyrinogen oxidase	-	Homo sapiens
coproporphyrinogen oxidase	-	Mus musculus
CPO	-	Homo sapiens
CPO	-	Mus musculus

Expression

Organism	Comment	Expression
Mus musculus	in subcutaneous A431 tumors in mice, pretreatment with vitamin D induces the expression of CCAAT-enhancer binding proteins (C/EBPbeta) isoforms, and of coproporphyrinogen oxidase (CPO). Vitamin D leads to strong accumulation of protoporphyrinogen-IX, PpIX. The murine CPO gene upstream region contains many competent C/EBP binding sites, analysis of C/EBPbeta and enzyme CPO interaction analysis, binding site analysis, overview. All C/EBP sites in murine CPO are asymmetric, with wide divergence between the two half-sites, fifteen functional C/EBP binding motifs in the mCPO promoter are found	up
Homo sapiens	pretreatment with vitamin D induces the expression of CCAAT-enhancer binding proteins (C/EBPbeta) isoforms, and of coproporphyrinogen oxidase (CPO). Vitamin D leads to strong accumulation of protoporphyrinogen-IX, PpIX. When MEL cells are incubated in 1% DMSO, large amounts of porphyrins are produced and under these conditions C/EBPbeta expression is upregulated. Concurrently, CPO expression is increased at both mRNA and protein levels, and higher levels of protoporphyrinogen-IX are generated in response to exogenously added 5-aminolevulic acid. LNCaP carcinoma lines respond to calcitriol with increases in enzyme CPO and protoporphyrinogen-IX levels concurrently. The human CPO gene upstream region contains many competent C/EBP binding sites, analysis of C/EBPbeta and enzyme CPO interaction analysis, binding site analysis, overview. Eighteen functional C/EBP binding motifs in the mCPO promoter are found, all are predicted to bind C/EBPs with moderate or higher affinity	up

General Information

General Information	Comment	Organism
metabolism	coproporphyrinogen oxidase (CPO) is an enzyme involved in the heme pathway responsible for the conversion of 5-aminolevulinic acid into protoporphyrin IX, coproporphyrinogen oxidase (CPO) is the sixth enzyme in the cascade	Homo sapiens
metabolism	coproporphyrinogen oxidase (CPO) is an enzyme involved in the heme pathway responsible for the conversion of 5-aminolevulinic acid into protoporphyrin IX, coproporphyrinogen oxidase (CPO) is the sixth enzyme in the cascade	Mus musculus
physiological function	coproporphyrinogen oxidase (CPO) is a rate-limiting enzyme in the heme pathway responsible for the conversion of coproporphyrinogen III into protoporphyrin IX	Mus musculus
physiological function	coproporphyrinogen oxidase (CPO) is a rate-limiting enzyme in the heme pathway responsible for the conversion of coproporphyrinogen III into protoporphyrin IX. The efficacy of photodynamic therapy for epithelial cancers is increased when photodynamic therapy is combined with calcitriol (Vit D), a form of differentiation therapy, underlying mechanism, overview. Differentiation therapy is associated with upregulation of C/EBPs, CPO gene expression, and PpIX production in tumors in vivo. Differentiation-promoting agents are known to upregulate CCAAT-enhancer binding proteins (C/EBPs), powerful regulators of cellular differentiation, and coproporphyrinogen oxidase (CPO). Cooperative interactions between regularly spaced C/EBP sites appear critical for CPO transcriptional regulation by differentiation therapy, mechanistic rationale for DT/PDT combination therapy for cancer	Homo sapiens

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)