Literature summary for 1.3.3.3 extracted from

Stephenson, J.R.; Thomas, N.E.; Friesen, J.A.; Jones, M.A.
Use of crosslinking to assess subunit interaction of recombinant human coproporphyrinogen oxidase (2005), Am. J. Biochem. Biotechnol., 1, 103-106.

No PubMed abstract available

Search for more literature for 1.3.3.3

Application

Application	Comment	Organism
additional information	functions as a homodimer in solution	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
6x-his-tagged enzyme expressed in Escherichia coli	Homo sapiens

Inhibitors

Inhibitors	Comment	Organism	Structure
bis(sulfosuccinimidyl) suberate	56% residual activity as compared to not cross-linked enzyme	Homo sapiens
sulfosuccinimidyl 4-(N-maleimidomethyl) cyclohexane-1-carboxylate	40% residual activity as compared to not cross-linked enzyme	Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
37500	-	1 * 37500, SDS-PAGE, absence of crosslinker	Homo sapiens
37500	-	2 * 37500, SDS-PAGE and cross-linking studies	Homo sapiens
86000	-	3 * 86000, SDS-PAGE, presence of crosslinker	Homo sapiens
107000	-	4 * 107000, SDS-PAGE, presence of crosslinker	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
by nickel affinity chromatography	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
coproporphyrinogen-III + O2 + 2 H+	-	Homo sapiens	protoporphyrinogen-IX + 2 CO2 + 2 H2O	-	?

Subunits

Subunits	Comment	Organism
dimer	2 * 37500, SDS-PAGE and cross-linking studies	Homo sapiens
homodimer	2 * 65000SDS-PAGE, presence of crosslinker	Homo sapiens
monomer	1 * 37500, SDS-PAGE, absence of crosslinker	Homo sapiens
tetramer	4 * 107000, SDS-PAGE, presence of crosslinker	Homo sapiens
trimer	3 * 86000, SDS-PAGE, presence of crosslinker	Homo sapiens

Synonyms

Synonyms	Comment	Organism
copro'gen oxidase	-	Homo sapiens

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Release 2023.1 (January 2023)