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Literature summary for 1.3.3.3 extracted from
- Molecular characterization of coproporphyrinogen oxidase from Glycine max and Arabidopsis thaliana (2002), Plant Physiol. Biochem., 40, 289-298.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene AtCPO-1, chromosome I, DNA and amino acid sequence determination and analysis, complementation of an enzyme-deletion Saccharomyces cerevisiae hem13 mutant, expression as GFP-fusion protein in transgenic Arabidopsis thaliana plants via Agrobacterium tumefaciens tranbsformation | Arabidopsis thaliana |
| gene AtCPO-2, DNA sequence determination and analysis, no complementation of an enzyme-deletion Saccharomyces cerevisiae hem13 mutant, possibly a pseudogene | Arabidopsis thaliana |
| over 200fold overexpression in Escherichia coli strain BL21(DE3), reduction of the toxicity of product protoporphyrin-IX for the bacterial cells by decrease of growth temperature to 20°C | Glycine max |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00025 | - |
Coproporphyrinogen-III | recombinant enzyme | Glycine max |  |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Arabidopsis thaliana | 9507 | - |
| etioplast | - |
Glycine max | 9513 | - |
| additional information | no activity in mitochondria | Glycine max | - |
- |
| additional information | no activity in mitochondrion and cytosol | Arabidopsis thaliana | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 37000 | - |
x * 37000, SDS-PAGE | Vigna radiata var. radiata |
| 37000 | - |
x * 37000, SDS-PAGE | Lotus japonicus |
| 37000 | - |
2 * 37000, recombinant and native wild-type enzyme, SDS-PAGE | Glycine max |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coproporphyrinogen-III + O2 | Glycine max | enzyme is involved in heme and chlorophyll biosynthesis | protoporphyrinogen-IX + CO2 + H2O | - |
? | |
| coproporphyrinogen-III + O2 | Arabidopsis thaliana | enzyme is involved in heme and chlorophyll biosynthesis | protoporphyrinogen-IX + CO2 + H2O | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | Q93Z96 | 2 genesAtCPO-1 and AtCPO-2, the latter being a pseudogene | - |
| Arabidopsis thaliana | Q9LR75 | 2 genesAtCPO-1 and AtCPO-2, the latter being a pseudogene | - |
| Glycine max | - |
- |
- |
| Lotus japonicus | - |
- |
- |
| Vigna radiata var. radiata | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant enzyme from Escherichia coli, 3-step procedure, 119fold to homogeneity | Glycine max |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Vigna radiata var. radiata | - |
| leaf | - |
Lotus japonicus | - |
| leaf | - |
Arabidopsis thaliana | - |
| root | - |
Arabidopsis thaliana | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 117.5 | - |
purified recombinant enzyme | Glycine max |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coproporphyrinogen-III + O2 | - |
Glycine max | protoporphyrinogen-IX + CO2 + H2O | - |
? | |
| coproporphyrinogen-III + O2 | - |
Arabidopsis thaliana | protoporphyrinogen-IX + CO2 + H2O | - |
? | |
| coproporphyrinogen-III + O2 | enzyme is involved in heme and chlorophyll biosynthesis | Glycine max | protoporphyrinogen-IX + CO2 + H2O | - |
? | |
| coproporphyrinogen-III + O2 | enzyme is involved in heme and chlorophyll biosynthesis | Arabidopsis thaliana | protoporphyrinogen-IX + CO2 + H2O | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 37000, SDS-PAGE | Vigna radiata var. radiata |
| ? | x * 37000, SDS-PAGE | Lotus japonicus |
| dimer | 2 * 37000, recombinant and native wild-type enzyme, SDS-PAGE | Glycine max |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| coproporphyrinogen III oxidase | - |
Vigna radiata var. radiata |
| coproporphyrinogen III oxidase | - |
Glycine max |
| coproporphyrinogen III oxidase | - |
Lotus japonicus |
| coproporphyrinogen III oxidase | - |
Arabidopsis thaliana |
| CPO | - |
Vigna radiata var. radiata |
| CPO | - |
Glycine max |
| CPO | - |
Lotus japonicus |
| CPO | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Arabidopsis thaliana |
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