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Literature summary for 1.3.3.12 extracted from

  • Logan, F.J.; Taylor, M.C.; Wilkinson, S.R.; Kaur, H.; Kelly, J.M.
    The terminal step in vitamin C biosynthesis in Trypanosoma cruzi is mediated by a FMN-dependent galactonolactone oxidase (2007), Biochem. J., 407, 419-426.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.3.3.12

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL-21+ cells Trypanosoma cruzi

Protein Variants

Protein Variants Comment Organism
H447G the mutant is inactive and shows undetectable FMN binding Trypanosoma cruzi
K450G the substitution results in an enzyme that retains an ability to bind FMN (around 50%of the wild type enzyme), but which exhibits no activity Trypanosoma cruzi
K55G the mutation has only a minor inhibitory effect on both FMN binding and biochemical activity Trypanosoma cruzi
K55H the mutation causes dramatically reduced FMN binding and enzyme activity (above 90%) Trypanosoma cruzi
K55L the mutant is inactive and shows undetectable FMN binding Trypanosoma cruzi
W448G the mutant is inactive and shows undetectable FMN binding Trypanosoma cruzi

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ complete inhibition at 1 mM Trypanosoma cruzi
N-ethylmaleimide 77% inhibition at 1 mM Trypanosoma cruzi
p-chloromercuribenzoate complete inhibition at 1 mM Trypanosoma cruzi
Zn2+ 63% inhibition at 1 mM Trypanosoma cruzi

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
10.82
-
 D-arabinono-1,4-lactone in 50 mM potassium phosphate buffer, pH 8.0, with 1 mM EDTA Trypanosoma cruzi
11.22
-
 L-galactono-1,4-lactone in 50 mM potassium phosphate buffer, pH 8.0, with 1 mM EDTA Trypanosoma cruzi

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
 SDS-PAGE Trypanosoma cruzi

Organism

Organism UniProt Comment Textmining
Trypanosoma cruzi
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Trypanosoma cruzi

Source Tissue

Source Tissue Comment Organism Textmining
amastigote
-
 Trypanosoma cruzi
-
epimastigote
-
 Trypanosoma cruzi
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinono-1,4-lactone + O2
-
 Trypanosoma cruzi ?
-
 ?
L-galactono-1,4-lactone + O2
-
 Trypanosoma cruzi L-ascorbate + H2O2
-
 ?

Synonyms

Synonyms Comment Organism
GAL
-
 Trypanosoma cruzi
galactonolactone oxidase
-
 Trypanosoma cruzi

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 heat-inactivated above 50°C Trypanosoma cruzi

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.161
-
 L-galactono-1,4-lactone in 50 mM potassium phosphate buffer, pH 8.0, with 1 mM EDTA Trypanosoma cruzi
0.285
-
 D-arabinono-1,4-lactone in 50 mM potassium phosphate buffer, pH 8.0, with 1 mM EDTA Trypanosoma cruzi

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8
-
 Trypanosoma cruzi

Cofactor

Cofactor Comment Organism Structure
FMN dependent on non-covalently-bound FMN Trypanosoma cruzi