Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Klebsiella pneumoniae |
Protein Variants | Comment | Organism |
---|---|---|
H84A | active site mutant with reduced activity | Klebsiella pneumoniae |
H84N | active site mutant with reduced activity | Klebsiella pneumoniae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
SDS-PAGE | Klebsiella pneumoniae |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Klebsiella pneumoniae | catalyzes the final step of PQQ formation, which involves a ring closure and an overall eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid in the absence of a redox-active metal or cofactor | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Klebsiella pneumoniae | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Q-Sepharose column chromatography and Ultrogel AcA54 gel filtration | Klebsiella pneumoniae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid + O2 | - |
Klebsiella pneumoniae | 4,5-dioxo-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate + H2O2 + H2O | - |
? | |
additional information | catalyzes the final step of PQQ formation, which involves a ring closure and an overall eight-electron oxidation of 3a-(2-amino-2-carboxyethyl)-4,5-dioxo-4,5,6,7,8,9-hexahydroquinoline-7,9-dicarboxylic acid in the absence of a redox-active metal or cofactor | Klebsiella pneumoniae | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PqqC | - |
Klebsiella pneumoniae |