Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.3.10 extracted from

  • Genet, R.; Donoyelle, C.; Menez, A.
    Purification and partial characterization of an amino acid alpha,beta-dehydrogenase, L-tryptophan 2',3'-oxidase from Chromobacterium violaceum (1994), J. Biol. Chem., 269, 18177-18184.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
5-hydroxy-L-tryptophan
-
Chromobacterium violaceum
D-tryptophan
-
Chromobacterium violaceum
methyl-3-indole
-
Chromobacterium violaceum
additional information no inhibition with L-histidine, N-acetyl-L-phenylalaninamide and N-acetyl-L-tyrosinamide Chromobacterium violaceum
tryptamine
-
Chromobacterium violaceum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0195
-
N-Acetyl-L-tryptophanamide
-
Chromobacterium violaceum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Chromobacterium violaceum 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14000
-
alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE Chromobacterium violaceum
74000
-
alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE Chromobacterium violaceum
680000
-
gel filtration Chromobacterium violaceum

Organism

Organism UniProt Comment Textmining
Chromobacterium violaceum
-
strain ATCC12472
-
Chromobacterium violaceum ATCC 12472
-
strain ATCC12472
-

Purification (Commentary)

Purification (Comment) Organism
cell disruption with Eaton press, ultracentrifugation, ammonium sulfate precipitation, DEAE-column, concentration using centrifugal microcentrators, gel filtration, purified 108fold, yield 33.8% Chromobacterium violaceum

Storage Stability

Storage Stability Organism
-80°C, for several months and for 24-48 h at room temperature in the absence of any additives Chromobacterium violaceum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
N-acetyl-L-tryptophanamide + O2
-
Chromobacterium violaceum N-acetyl-alpha,beta-didehydrotryptophanamide + H2O2
-
?
N-acetyl-L-tryptophanamide + O2
-
Chromobacterium violaceum ATCC 12472 N-acetyl-alpha,beta-didehydrotryptophanamide + H2O2
-
?

Subunits

Subunits Comment Organism
octamer alpha, 4 * 14000 + beta 4 * 74000, organized in an alpha4,beta4 manner, heterooligomeric structure, one heme molecule per alpha,beta protomer, SDS-PAGE Chromobacterium violaceum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
Chromobacterium violaceum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.753
-
N-Acetyl-L-tryptophanamide
-
Chromobacterium violaceum

pH Range

pH Minimum pH Maximum Comment Organism
3 9.5
-
Chromobacterium violaceum

Cofactor

Cofactor Comment Organism Structure
additional information NAD+, NADP+, FAD, or FMN show no effect on the activity Chromobacterium violaceum

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.0013
-
methyl-3-indole
-
Chromobacterium violaceum
0.0054
-
tryptamine
-
Chromobacterium violaceum
0.0066
-
D-tryptophan
-
Chromobacterium violaceum
0.037
-
5-hydroxy-L-tryptophan
-
Chromobacterium violaceum