Cloned (Comment) | Organism |
---|---|
gene GLDH, quantitative real-time RT-PCR enzyme expression analysis | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | homozygous Arabidopsis thaliana mutant (gldhRNAi3-11) plants with approximately 40% of the GLDH activity of wild-type controls are developed by RNA interference (three genotypes), and are viable under standard laboratory conditions. Mutant gldhRNAi3-11 plants show about 20% decrease in the contents of reduced ascorbic acid and total ascorbic acid. Partial suppression of GLDH activity in RNAi lines confers significant reduction in leaf water loss through decreasing stomatal aperture size in Arabidopsis thaliana, phenotype, overview | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | - |
Arabidopsis thaliana | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + 4 ferricytochrome c | Arabidopsis thaliana | overall reaction | L-dehydroascorbate + 4 ferrocytochrome c + 4 H+ | - |
? | |
L-galactono-1,4-lactone + 4 ferricytochrome c | Arabidopsis thaliana Col-0 | overall reaction | L-dehydroascorbate + 4 ferrocytochrome c + 4 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9SU56 | - |
- |
Arabidopsis thaliana Col-0 | Q9SU56 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
rosette leaf | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + 4 ferricytochrome c | overall reaction | Arabidopsis thaliana | L-dehydroascorbate + 4 ferrocytochrome c + 4 H+ | - |
? | |
L-galactono-1,4-lactone + 4 ferricytochrome c | overall reaction | Arabidopsis thaliana Col-0 | L-dehydroascorbate + 4 ferrocytochrome c + 4 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GLDH | - |
Arabidopsis thaliana |
L-galactono-1,4-lactone dehydrogenase | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | - |
Arabidopsis thaliana | |
FAD | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
malfunction | homozygous Arabidopsis thaliana mutant gldhRNAi3-11 plants show approximately 40% of the GLDH activity of wild-type controls and are viable under standard laboratory conditions. Mutant gldhRNAi3-11 plants show about 20% decrease in the contents of reduced ascorbic acid and total ascorbic acid. Partial suppression of GLDH activity confers significant reduction in leaf water loss through decreasing stomatal aperture size in Arabidopsis thaliana, phenotype, overview | Arabidopsis thaliana |