Cloned (Comment) | Organism |
---|---|
gene GLDH, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant 19fold overexpression in Lactuca sativa under the control of the pea plastocyanin promoter and via Agrobacterium tumefcáciens strain LBA4404 mediated transformation, semi-quantitative and quantitative real-time RT-PCR enzyme expression analysis. Transformation experiments in lettuce with gene under the control of the CaMV 35S promoter fails | Lactuca sativa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | the enzyme contains a mitochondrial targeting sequence | Lactuca sativa | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + 4 ferricytochrome c | Lactuca sativa | overall reaction | L-dehydroascorbate + 4 ferrocytochrome c + 4 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Lactuca sativa | W6N8V9 | cv. Iceberg | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Lactuca sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + 4 ferricytochrome c | overall reaction | Lactuca sativa | L-dehydroascorbate + 4 ferrocytochrome c + 4 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 69200, about, preprotein, sequence calculation, x * 57200, mature protein, sequence calculation | Lactuca sativa |
Synonyms | Comment | Organism |
---|---|---|
L-galactono-1,4-lactone dehydrogenase | - |
Lactuca sativa |
L-GalLDH | - |
Lactuca sativa |
LsL-GalLDH | - |
Lactuca sativa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | - |
Lactuca sativa | |
FAD | a putative N-terminal FAD-binding domain localizes between residues 128 and 264, wherein is located a motif 157VGSGLSP163 common to L-GalLDHs characterized from plants | Lactuca sativa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Lactuca sativa | sequence calculation, mature protein | - |
6.68 |
Lactuca sativa | sequence calculation, preprotein | - |
8.68 |
General Information | Comment | Organism |
---|---|---|
metabolism | L-galactono-1,4-lactone dehydrogenase is one of the enzymes of the Smirnoff-Wheeler's branch of ascorbic acid biosynthetic pathway, overview | Lactuca sativa |