Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.3.2.3 extracted from

  • Landi, M.; Fambrini, M.; Basile, A.; Salvini, M.; Guidi, L.; Pugliesi, C.
    Overexpression of L-galactono-1,4-lactone dehydrogenase (L-GalLDH) gene correlates with increased ascorbate concentration and reduced browning in leaves of Lactuca sativa L. after cutting (2015), Plant Cell Tissue Organ Cult., 123, 109-120 .
No PubMed abstract available
Search for more literature for 1.3.2.3

Cloned(Commentary)

Cloned (Comment) Organism
gene GLDH, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant 19fold overexpression in Lactuca sativa under the control of the pea plastocyanin promoter and via Agrobacterium tumefcáciens strain LBA4404 mediated transformation, semi-quantitative and quantitative real-time RT-PCR enzyme expression analysis. Transformation experiments in lettuce with gene under the control of the CaMV 35S promoter fails Lactuca sativa

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion the enzyme contains a mitochondrial targeting sequence Lactuca sativa 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-galactono-1,4-lactone + 4 ferricytochrome c Lactuca sativa overall reaction L-dehydroascorbate + 4 ferrocytochrome c + 4 H+
-
 ?

Organism

Organism UniProt Comment Textmining
Lactuca sativa W6N8V9 cv. Iceberg
-

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
 Lactuca sativa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-galactono-1,4-lactone + 4 ferricytochrome c overall reaction Lactuca sativa L-dehydroascorbate + 4 ferrocytochrome c + 4 H+
-
 ?

Subunits

Subunits Comment Organism
? x * 69200, about, preprotein, sequence calculation, x * 57200, mature protein, sequence calculation Lactuca sativa

Synonyms

Synonyms Comment Organism
L-galactono-1,4-lactone dehydrogenase
-
 Lactuca sativa
L-GalLDH
-
 Lactuca sativa
LsL-GalLDH
-
 Lactuca sativa

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
 Lactuca sativa
FAD a putative N-terminal FAD-binding domain localizes between residues 128 and 264, wherein is located a motif 157VGSGLSP163 common to L-GalLDHs characterized from plants Lactuca sativa

pI Value

Organism Comment pI Value Maximum pI Value
Lactuca sativa sequence calculation, mature protein
-
 6.68
Lactuca sativa sequence calculation, preprotein
-
 8.68

General Information

General Information Comment Organism
metabolism L-galactono-1,4-lactone dehydrogenase is one of the enzymes of the Smirnoff-Wheeler's branch of ascorbic acid biosynthetic pathway, overview Lactuca sativa