Cloned (Comment) | Organism |
---|---|
gene L-GalLDH1, quantitative real-time PCR isozyme expression analysis | Oryza sativa Japonica Group |
gene L-GalLDH2, quantitative real-time PCR isozyme expression analysis | Oryza sativa Japonica Group |
Protein Variants | Comment | Organism |
---|---|---|
additional information | significant differences in grain chalkiness degree are observed between mutant GI and WT. GI-1 kernels display an increased (25.78%) grain chalkiness degree, as well as GI-2 kernels (16.33%), but wild-type kernels display 9.37%. In addition, the GI-1 and GI-2 grains show a lower starch content when compared with wild-type , which is decreased to 51.06% and 82.64% of the wild-type level, respectively. Similarly, significant lower amylose content is also observed in GI-1 and GI-2 grains (3.08% and 3.90%) when compared with wild-type (5.12%). As chalkiness may be affected by grain size, grain length, grain width, and grain length-to-width ratio (GLWR) of GI, and wild-type are measured, with the results showing that no significant difference between GI-1, GI-2 and wild-type in grain length, grain width, or GLWR is observed. Phenotypes, overview | Oryza sativa Japonica Group |
additional information | significant differences in grain chalkiness degree are observed between mutant GI and WT. GI-1 kernels display an increased (25.78%) grain chalkiness degree, as well as GI-2 kernels (16.33%), but wild-type kernels display 9.37%. In addition, the GI-1 and GI-2 grains show a lower starch content when compared with wild-type, which is decreased to 51.06% and 82.64% of the wild-type level, respectively. Similarly, significant lower amylose content is also observed in GI-1 and GI-2 grains (3.08% and 3.90%) when compared with wild-type (5.12%). As chalkiness may be affected by grain size, grain length, grain width, and grain length-to-width ratio (GLWR) of GI, and wild-type are measured, with the results showing that no significant difference between GI-1, GI-2 and wild-type in grain length, grain width, or GLWR is observed. Phenotypes, overview | Oryza sativa Japonica Group |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | L-GalLDH is attached to complex I of the mitochondrial electron transport chain | Oryza sativa Japonica Group | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + 4 ferricytochrome c | Oryza sativa Japonica Group | overall reaction | L-dehydroascorbate + 4 ferrocytochrome c + 4 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryza sativa Japonica Group | Q2QXY1 | cv. Zhonghua 11 | - |
Oryza sativa Japonica Group | Q2RAP0 | cv. Zhonghua 11 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kernel | - |
Oryza sativa Japonica Group | - |
leaf | - |
Oryza sativa Japonica Group | - |
seed | - |
Oryza sativa Japonica Group | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + 4 ferricytochrome c | overall reaction | Oryza sativa Japonica Group | L-dehydroascorbate + 4 ferrocytochrome c + 4 H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
L-galactono-1,4-lactone dehydrogenase | - |
Oryza sativa Japonica Group |
L-GalLDH | - |
Oryza sativa Japonica Group |
L-GalLDH1 | - |
Oryza sativa Japonica Group |
L-GalLDH2 | - |
Oryza sativa Japonica Group |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
2 | assay at | Oryza sativa Japonica Group |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Oryza sativa Japonica Group |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
cytochrome c | - |
Oryza sativa Japonica Group |
General Information | Comment | Organism |
---|---|---|
malfunction | ascorbic acid deficiency in L-GalLDH-suppressed transgenic rice, GI-1 and GI-2, which have constitutively low (between 30% and 50%) leaf and grain ascorbic acid content compared with the wild-type, leads to increased grain chalkiness in the transgenic rice. Deficiency of ascorbic acid also results in a higher lipid peroxidation and H2O2 content, accompanied by a lower hydroxyl radical scavenging rate, total antioxidant capacity and photosynthetic ability. Changes of the enzyme activities and gene transcript abundances related to starch synthesis are also observed in GI-1 and GI-2 grains. Phenotypes, detailed overview | Oryza sativa Japonica Group |
physiological function | L-galactono-1,4-lactone dehydrogenase catalyzes the ultimate step of ascorbic acid biosynthesis in higher plants. L-GalLDH is attached to complex I of the mitochondrial electron transport chain, which uses L-galactono-1,4-lactone as an electron donor to reduce cytochrome c between complexes III and IV, while l-GalLis converted into ascorbic acid. Role of L-GalLDH in the control of cell, organ, and plant growth | Oryza sativa Japonica Group |
physiological function | L-galactono-1,4-lactone dehydrogenase catalyzes the ultimate step of ascorbic acid biosynthesis in higher plants. L-GalLDH is attached to complex I of the mitochondrial electron transport chain,which uses L-galactono-1,4-lactone as an electron donor to reduce cytochrome c between complexes III and IV, while l-GalLis converted into ascorbic acid. Role of L-GalLDH in the control of cell, organ, and plant growth | Oryza sativa Japonica Group |