Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Nicotiana tabacum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Acriflavine | diminished by addition of FAD or FMN | Nicotiana tabacum | |
Cu2+ | strong inhibition | Nicotiana tabacum | |
N-ethylmaleimide | complete inhibition | Nicotiana tabacum | |
p-chloromercuribenzoate | - |
Nicotiana tabacum | |
Zn2+ | strong inhibition | Nicotiana tabacum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | soluble | Nicotiana tabacum | 5739 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
recombinant enzyme, sequence determination | Nicotiana tabacum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + 2 ferricytochrome c | Nicotiana tabacum | - |
L-ascorbate + 2 ferrocytochrome c + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nicotiana tabacum | - |
tobacco | - |
Purification (Comment) | Organism |
---|---|
partial, wild-type and recombinant from Escherichia coli | Nicotiana tabacum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Nicotiana tabacum | - |
root | - |
Nicotiana tabacum | - |
stem | - |
Nicotiana tabacum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-galactono-1,4-lactone + 2 ferricytochrome c | - |
Nicotiana tabacum | L-ascorbate + 2 ferrocytochrome c + 2 H+ | - |
? | |
L-galactono-1,4-lactone + ferricytochrome c | native and recombinant enzyme | Nicotiana tabacum | L-ascorbate + ferrocytochrome c | - |
? | |
L-galactono-1,4-lactone + ferricytochrome c | specific for L-galactono-gamma-lactone | Nicotiana tabacum | L-ascorbate + ferrocytochrome c | - |
? | |
L-gulono-gamma-lactone + ferricytochrome | recombinant enzyme: 7% activity of the activity with L-galactono-gamma-lactone | Nicotiana tabacum | D-ascorbate + ferricytochrome c | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 56000, SDS-PAGE | Nicotiana tabacum |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | - |
Nicotiana tabacum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
flavin | - |
Nicotiana tabacum |