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Literature summary for 1.3.2.3 extracted from

  • Oba, K.; Ishikawa, S.; Nishikawa, M.; Mizuno, H.; Yamamoto, T.
    Purification and properties of L-galactono-gamma-lactone dehydrogenase, a key enzyme for ascorbic acid biosynthesis, from sweet potato roots (1995), J. Biochem., 117, 120-124.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
Acriflavine diminished by addition of FAD or FMN Ipomoea batatas
L-galactono-gamma-lactone substrate inhibition at concentration above 4.2 mM Ipomoea batatas
p-chloromercuribenzoate reduced by addition of 2-mercapthoethanol Ipomoea batatas

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
L-galactono-gamma-lactone
-
Ipomoea batatas

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Ipomoea batatas 5739
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
56000
-
gel filtration Ipomoea batatas

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
L-galactono-1,4-lactone + 2 ferricytochrome c Ipomoea batatas terminal step in L-ascorbic acid biosynthesis L-ascorbate + 2 ferrocytochrome c + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Ipomoea batatas
-
sweet potato
-

Purification (Commentary)

Purification (Comment) Organism
low recovery Ipomoea batatas

Source Tissue

Source Tissue Comment Organism Textmining
root
-
Ipomoea batatas
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
37000
-
purified enzyme Ipomoea batatas

Storage Stability

Storage Stability Organism
-80°C, 0.3 mg per ml protein, 0.1 M potassium phosphate, pH 7.4, 10% glycerol,2 mM glutathione, 2 month Ipomoea batatas
-80°C, diluted protein, 0.1 M potassium phosphate, pH 7.4, 10% glycerol,2 mM glutathione, 30% loss of activity after 1 month Ipomoea batatas

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
L-galactono-1,4-lactone + 2 ferricytochrome c terminal step in L-ascorbic acid biosynthesis Ipomoea batatas L-ascorbate + 2 ferrocytochrome c + 2 H+
-
?
L-galactono-1,4-lactone + ferricytochrome c specific for L-galactono-gamma-lactone Ipomoea batatas L-ascorbate + ferrocytochrome c
-
?
L-gulono-gamma-lactone + ferricytochrome very low activity Ipomoea batatas D-ascorbate + ferricytochrome c
-
?

Subunits

Subunits Comment Organism
monomer 1 * 56000, SDS-PAGE Ipomoea batatas

Synonyms

Synonyms Comment Organism
GLDHase
-
Ipomoea batatas

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
27
-
assay at Ipomoea batatas

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.9
-
-
Ipomoea batatas

Cofactor

Cofactor Comment Organism Structure
flavin probably Ipomoea batatas