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Literature summary for 1.3.1.97 extracted from

  • Bell, S.A.; Niehaus, T.D.; Nybo, S.E.; Chappell, J.
    Structure-function mapping of key determinants for hydrocarbon biosynthesis by squalene and squalene synthase-like enzymes from the green alga Botryococcus braunii race B (2014), Biochemistry, 53, 7570-7581 .
    View publication on PubMed
Search for more literature for 1.3.1.97

Cloned(Commentary)

Cloned (Comment) Organism
gene SSL-3, sequence comparisons, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Botryococcus braunii

Protein Variants

Protein Variants Comment Organism
N171A/G207Q site-directed mutagenesis, combined N171A and G207Q mutations in the SSL-3 backbone result in complete conversion of SSL-3 from an enzyme that used presqualene diphosphate for botryococcene biosynthesis to one that used presqualene diphosphate for squalene biosynthesis Botryococcus braunii
Y166A site-directed mutagenesis, mutant substrate specificity and activity compared to the wild-type Botryococcus braunii
Y166F site-directed mutagenesis, mutant substrate specificity and activity compared to the wild-type Botryococcus braunii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
presqualene diphosphate + NADPH + H+ Botryococcus braunii mutant SSL-3 squalene + diphosphate + NADP+
-
 ?
presqualene diphosphate + NADPH + H+ Botryococcus braunii
-
 C30 botryococcene + diphosphate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Botryococcus braunii G0Y288 race B
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with farnesyl diphosphate. Analysis of substrate specificity, overview Botryococcus braunii ?
-
 ?
presqualene diphosphate + NADPH + H+ mutant SSL-3 Botryococcus braunii squalene + diphosphate + NADP+
-
 ?
presqualene diphosphate + NADPH + H+
-
 Botryococcus braunii C30 botryococcene + diphosphate + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
SSL-3
-
 Botryococcus braunii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Botryococcus braunii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.3
-
 assay at Botryococcus braunii

Cofactor

Cofactor Comment Organism Structure
NADPH
-
 Botryococcus braunii

General Information

General Information Comment Organism
additional information proposed catalytic cascades for the enzyme-mediated biosynthesis of squalene and botryococcene, and molecular modeling of Botryococcus braunii botryococcene and squalene synthase enzymes, overview. Substrate docking and molecular modeling Botryococcus braunii