Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Botryococcus braunii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
squalestatin | - |
Botryococcus braunii | |
Triton X-100 | - |
Botryococcus braunii | |
Tween 80 | enzyme activity is completely abolished by 0.5% (v/v) Tween 80 | Botryococcus braunii |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Botryococcus braunii | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required for activity | Botryococcus braunii | |
additional information | no enzyme activity is evident with Co2+ or Mn2+ as a cofactor | Botryococcus braunii |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52500 | - |
x * 52500, estimated from amino acid sequence and SDS-PAGE | Botryococcus braunii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Botryococcus braunii | - |
race B | - |
Botryococcus braunii Berkeley (Showa) | - |
race B | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
presqualene diphosphate + NADPH + H+ | - |
Botryococcus braunii | C30 botryococcene + NADP+ + diphosphate | - |
? | |
presqualene diphosphate + NADPH + H+ | - |
Botryococcus braunii Berkeley (Showa) | C30 botryococcene + NADP+ + diphosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52500, estimated from amino acid sequence and SDS-PAGE | Botryococcus braunii |
Synonyms | Comment | Organism |
---|---|---|
botryococcene synthase | - |
Botryococcus braunii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Botryococcus braunii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
in MOPS buffer | Botryococcus braunii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Botryococcus braunii |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.000001 | - |
squalestatin | at pH 7.3 and 37°C | Botryococcus braunii |
Organism | Comment | Expression |
---|---|---|
Botryococcus braunii | gene expression is preferential during rapid growth. Enzyme activity is initially low in the cells, but rises greater than 10fold to a maximum by day 3, and then decreases gradually to almost undetectable levels by the end of the culture period | up |